Bacteria and Fungus Identification
Bacterial & Fungal
Identification
MicroSEQ® Microbial Identification System
Harmful bacteria and fungi can contaminate your raw materials, production facilities and, even worse, your branded products.
That is why Applied Biosystems developed the MicroSEQ® Microbial Identification System based on rDNA sequencing. This rapid molecular system is the fastest and most accurate identification system for bacteria and fungi.
Go Molecular. The Answer Is in the DNA.
Used in top pharmaceutical companies worldwide, the MicroSEQ ID System is ideal for environmental monitoring and microbial identification in small molecule and biopharmaceutical manufacturing, and service laboratories.
MicroSEQ® Microbial Identification System
It can also be used in public health laboratories, cosmetics and personal care products manufacturing, food testing laboratories, and academic and research centers.
- Accurate genotypic bacterial identification based on the 16S rRNA gene
- Accurate genotypic fungal identification based on the D2 region of the 26S rRNA gene
- Easy workflow, high throughput, accurate results in five hours
- Fully validated and implemented in four months
- Enables 21 CFR Part 11 compliance
Workflow: Find the Products You Need for Every Step
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Step 1: Sample from Colony
After growing a culture of the bacteria or fungi you want to identify, sample cells from the colony.
Step 2: Extract DNA
Extract DNA from the bacteria or fungi in the colony or culture. The ideal colony size is 1-2mm for bacteria and 3mm for fungi.
Products for Step 2: Extract DNA
Reagents & Kits
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PrepMan® Ultra Sample Preparation Reagent
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Ideal for preparing DNA templates for PCR from bacteria and fungi.
Step 4: Sequence DNA
Next, sequence your sample DNA automatically by running it on a genetic analyzer (DNA sequencer). Choose from several different system configurations to meet your throughput needs.
Products for Step 4: Sequence DNA
Instruments & Accessories
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3130xl Genetic Analyzer
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High Throughput - 16-capillary throughput and advanced automation capabilities for laboratories that need streamlined set-up and 24-hour unattended operation.
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3130 Genetic Analyzer
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Medium Throughput - 4-capillary automated electrophoresis instrument that helps save time, reduce costs, and increase productivity
Step 5: Identify Organism
Use the MicroSEQ® ID Analysis Software to match sequences in your sample with sequences in our validated libraries. The software also enables you to create custom libraries and search public libraries.
Products for Step 5: Identify Organism
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Don't see the species of bacteria or fungi you are looking for in our libraries?
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Software
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MicroSEQ® ID Analysis Software
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Easy-to-use software that enables you to identify and classify bacterial or fungal sequences by comparing them to a validated microbial library, including Base Pair, Full Gene and Fungal libraries.
IQ/OQ Service
Overcome critical implementation challenges in the qualification of instrument and operation quality (IQ/OQ) through a collaborative partnership with Applied Biosystems.
- Consultation with client to determine needs
- Statement of work to define project
- Detailed verification test plan and documentation
- System installation verification
- Sequence of system operations qualification
- Microbial identification verification
- Accuracy report
- System software installation and security verification
- Software exporting, importing, archiving and verification
- Phylogenetic tree and custom library verifications
- Detailed data analysis and review
- Consultation on experimental process and results
- Performance Qualification (PQ) recommended guidelines
Implementation ProgramCustomize laboratory design and training to the specific needs of your lab, with the aim of completing Performance Qualification (PQ) within the six-month timeframe of the program.
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