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Bacterial Artificial Chromosome (BAC) Fingerprinting

Figure 1: Electropherogram of BAC fingerprinting data

Fingerprinting of large insert genomic fragment libraries, also known as BAC (bacterial artificial chromosomes) clones leads to the construction of a genome-wide physical map. These maps are critical to genome sequencing, positional cloning and understanding the relative organization of genes and markers. When BAC libraries are arranged into maps that reflect the DNA sequence in a chromosome, they provide maximal information and utility.

Electropherogram of BAC fingerprinting data (Figure 1) analyzed on an Applied Biosystems Genetic Analyzer. Data was analyzed by sizing the restriction enzyme digested fragments with GeneMapper Software.

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