Fast Resequencing

Step 1: DNA Purification

Products for Step 1: DNA Purification

MELT™ Total Nucleic Acid Isolation System

Stop homogenizing tissue samples. With the MELT Total RNA Isolation System, tissue samples are quickly and easily lysed in MELT solution. The RNA in the resulting lysate is stable for days at room temperature or longer at lower temperatures.

MagMAX™ Total Nucleic Acid Isolation Kit

For rapid high throughput purification of DNA and RNA for use in PCR and RT-PCR applications. Suitable for difficult-to-lyse samples, such as Mycobacterium.

Step 2: PCR Amplification & Cleanup

Products for Step 2: PCR Amplification & Cleanup

VariantSEQr™ Primers

This PCR-based resequencing system enables you to identify sequence variations in hundreds of disease-related human genes quickly and easily.

Veriti™ Thermocycler Systems

The Veriti® Thermal Cycler product line delivers the proven performance and reliability of Applied Biosystems thermal cyclers. The Veriti Thermal Cycler is now available in 96-well (choose between 0.1 and 0.2 mL), 384-well, and 60-well (0.5 ml) block formats to suit your thermal cycling needs.

AmpliTaq Gold® Fast PCR Master Mix, UP

Optimized for use in sequencing applications, this kit provides specific, high-yield amplicons that can be easily and robustly sequenced. This premixed master mix works with your existing primers to provide sensitive, specific and reproducible results.

Step 3: Cycle Sequencing

Products for Step 3: Cycle Sequencing

Veriti™ Thermocycler Systems

The Veriti® Thermal Cycler product line delivers the proven performance and reliability of Applied Biosystems thermal cyclers. The Veriti Thermal Cycler is now available in 96-well (choose between 0.1 and 0.2 mL), 384-well, and 60-well (0.5 ml) block formats to suit your thermal cycling needs.

BigDye® Terminator v1.1 Cycle Sequencing Kits

BigDye® Terminator v1.1 Cycle Sequencing Kits are designed for specialty applications that require optimal basecalling adjacent to the primer and for sequencing short PCR product templates with rapid electrophoresis run modules.

Step 4: Sequencing Reaction Cleanup

Products for Step 4: Sequencing Reaction Cleanup

BigDye XTerminator® Purification Kit

The BigDye XTerminator® Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye® terminators. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

Step 5: Capillary Electrophoresis

Products for Step 5: Capillary Electrophoresis

3500 Genetic Analyzer

The 3500 8-capillary platform can run a wide variety of applications—including de novo sequencing and resequencing (mutational profiling)—as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP® and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.

3500xL Genetic Analyzer

The 3500xL 24-capillary platform can run a wide variety of applications, including de novo sequencing and resequencing (mutational profiling), as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP® and SNP validation or screening. The majority of applications can be run on a single polymer and capillary array.

3730 DNA Analyzer

The 48-capillary 3730 DNA Analyzer is the Gold Standard in medium-to-high throughput genetic analysis.

3730xl DNA Analyzer

The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample.

3130 Genetic Analyzer

Applied Biosystems 3130 Genetic Analyzer is the latest generation of 4-capillary electrophoresis instruments for the low to medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity.

3130xl Genetic Analyzer

Applied Biosystems 3130xl Genetic Analyzer is the latest generation of 16-capillary electrophoresis instruments for the medium throughput laboratories.

310 Genetic Analyzer

The ABI PRISM® 310 Genetic Analyzer is an automated single-capillary genetic analyzer designed for a wide range of sequencing and fragment analysis applications.

Step 6: Data Analysis

Products for Step 6: Data Analysis

Variant Reporter™ Software

The Variant Reporter™ Software performs comparative sequencing, also known as direct sequencing, medical sequencing, PCR sequencing and resequencing with DNA sequencing files. The software is designed for reference based and non-reference based analysis such as mutation detection and analysis, SNP discovery and validation and sequence confirmation.

Sequencing Analysis Software

This software enables you to basecall, trim, display, edit, and print data from our entire line of capillary DNA sequencing instruments for data analysis and quality control.