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ISSR (Inter-Simple Sequence Repeat) Analysis

Inter-simple sequence repeat (ISSR) PCR is a fast, inexpensive genotyping technique based on variation in the regions between microsatellites. This method has a wide range of uses, including the characterization of genetic relatedness among populations, genetic fingerprinting, gene tagging, detection of clonal variation, cultivar identification, phylogenetic analysis, detection of genomic instability, and assessment of hybridization.

Figure 1

Figure 1: ISSR Genotyping

ISSR genotyping of endangered plants using an optimized workflow (Figure 1). Applied Biosystems, Inc. (2010)

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