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  7. MSMSA (Methylation Sensitive Mobility Shift Assay)

MSMSA (Methylation Sensitive Mobility Shift Assay)

MSMSA is a DNA fragment analysis application used to assess the degree of methylation present in a given amplicon. Samples from a fragment analysis assay can also be used in a downstream sequencing step, making this application ideal for screening. The assay primers are designed like bisulfite sequencing primers (ie: designed to regions without CpGs) using Methyl Primer Express Software, so that PCR amplification is not dependent on methylation status.

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