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  9. Sizing in Fragment Analysis

Sizing in Fragment Analysis

Fragment Sizing Analysis
Fragment Sizing Analysis Data
GeneScan™ Size Standards are fluorescently-labeled DNA fragments required for performing fragment analysis and genotyping applications on AB’s capillary electrophoresis platforms. They are added to the fragment sizing analysis experiments and ran in parallel to samples. Size standards and sample fragments loaded in the same capillary run undergo the same electrophoretic forces. Distance migrated depends on size of DNA samples.

Each standard contains fragments of known sizes that will be used by the data analysis software (GeneMapper) to generate a calibration curve, which is used to determine the size of unknown fragments in an experimental sample. GenScan™ size Standards generate reliable and reproducible size calls, and are available with either ROX™, TAMRA fluorophore or the proprietary 5th dye LIZ®. The LIZ® dye-labeled standards offer increased throughput and reduced project costs by enabling an increase in the number of markers that can be multiplexed in a single sample.

To learn more about our GeneScan Size standards used in sizing experiments please see our table below.



Sizing Information

When DNA sample fragments are labeled with: Choose a size standards labeled with: AB Dye Set: Possible Applications Other AB kits and products:
dR110, dR6G, dTAMRA™, dROX LIZ DS-02 SNaPshot® SNaPshot® primer focus kits, SNaPshot® Multiplex kits
5-FAM™, HEX™, NED™ ROX™ DS-30   Custom Oligos
6-FAM™, VIC™, NED™ ROX™ DS-31 Microsatellites (linkage mapping sets) LMS v2.5, Mouse Mapping Primers, Custom Oligos
5-FAM™, JOE, NED™ ROX™ DS-32 Microsatellite (Forensic) AmpFISTR Profiler Plus®, Cofiler, SGM Plus Kits, StockMarks Kits, Plant/Microbial AFLP Kits®
6-FAM™, VIC™, NED™, PET® LIZ DS-33 Microsatellite (Forensic) AmpFISTR Identifier Kits, AmpFISTR Yflir Amplification Kits, Custom Oligos, LMS v2.5, Mouse Mapping Primers, StockMarks Kits
6-FAM™, TET, HEX™ TAMRA DS-34    
6-FAM™, dR6G LIZ DS-40 SNP Genotyping SNPlex™ Genotyping System