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Reagents & Kits

  • Find which sequencing and reaction clean-up kits are right for you.


Which Sequencing Kit Is Right for You?


Application BigDye® Direct BigDye® Terminators v3.1 BigDye® Terminators v1.1 dRhodamine Terminators dGTP BigDye® Terminators
G-C Rich >65% +++ +++ +++ + +++
A-T Rich >65% +++ +++ +++ ++ ++
Homopolymer
A-T Rich >25 bp		 ++ ++ ++ ++ ++
GT Rich or Difficult Templates +++ +++ +++ ++ +++
de novo Sequencing
High Throughput (cDNA)		 + +++ ++ ++ ++
de novo Sequencing
Low-Mid Throughput		 + +++ ++ ++ ++
Sequence Specific Primers   +++ +++ +++ +++
M13 primers +++ +++ +++ +++ +++
Comparative Sequencing
(Germline Mutations, 50:50)		 +++ ++ +++ ++ ++
Comparative Sequencing
(Somatic Mutations, 10:50)		 +++ ++ +++ ++ ++
Resequencing Sequence Specific Primers   ++ +++    
Resequencing Universal Primers +++ ++ +++    
Long-Read Sequencing + +++ ++ ++ ++
Mixed-base detection +++ +++ +++ +++ +++
Fast capillary electrophoresis and high resolution sequence close to the sequencing primer +++ + +++ + +
BACs and Genomic DNA   +++ +++ ++ ++
+++ Optimal    ++ Recommended    + Satisfactory
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