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Reagents & Kits

  • Find which sequencing and reaction clean-up kits are right for you.

  • Sequencing Kits


    Which Sequencing Kit Is Right for You?


    Application BigDye® Direct BigDye® Terminators v3.1 BigDye® Terminators v1.1 dRhodamine Terminators dGTP BigDye® Terminators
    G-C Rich >65% +++ +++ +++ + +++
    A-T Rich >65% +++ +++ +++ ++ ++
    Homopolymer
    A-T Rich >25 bp    		 ++ ++ ++ ++ ++
    GT Rich or Difficult Templates +++ +++ +++ ++ +++
    de novo Sequencing
    High Throughput (cDNA)    		 + +++ ++ ++ ++
    de novo Sequencing
    Low-Mid Throughput    		 + +++ ++ ++ ++
    Sequence Specific Primers   +++ +++ +++ +++
    M13 primers +++ +++ +++ +++ +++
    Comparative Sequencing
    (Germline Mutations, 50:50)    		 +++ ++ +++ ++ ++
    Comparative Sequencing
    (Somatic Mutations, 10:50)    		 +++ ++ +++ ++ ++
    Resequencing Sequence Specific Primers   ++ +++    
    Resequencing Universal Primers +++ ++ +++    
    Long-Read Sequencing + +++ ++ ++ ++
    Mixed-base detection +++ +++ +++ +++ +++
    Fast capillary electrophoresis and high resolution sequence close to the sequencing primer +++ + +++ + +
    BACs and Genomic DNA   +++ +++ ++ ++
    +++ Optimal    ++ Recommended    + Satisfactory

  • Sequencing Primers


    Which Sequencing Primers Are Right for You?


    ApplicationPrimer Design Systems & Software
    Identify sequence variations in human disease-related genes
    Identify mitochodrial DNA sequence variations
    Methylation Analysis

  • Sequencing Clean-Up Kits


    Which Sample Cleanup Product Is Right for You?


    Cleanup MethodDescription & ApplicationProduct
    Reagent-Based Sequencing Template CleanupScavenges all unincorporated BigDye® terminators. Stabilizes samples before analysis. Best for long and short fragment recovery.
    Gel Filtration Spin ColumnsRecovers DNA fragments larger than 16 base pairs. Removes > 98% of salts, NTP's and other unwanted low-molecular-weight impurities.
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