The Attune® Acoustic Focusing Cytometer—now available in blue/red laser configuration

Choose the Laser Configuration for Your Specific Needs

The Blue/Red Configuration
Use this configuration with your current blue/red reagents that you are already familiar with. It delivers the same precision and sensitivity as the blue/violet configuration for many standard research applications. For example, the blue/red configuration shows excellent segregation of populations in immunophenotyping experiments with up to six colors (Figure 1).


Figure 1. Six-color immunophenotyping analysis on the Attune® Acoustic Focusing Cytometer with blue/red laser configuration. Mouse (C57BL/6J) peripheral blood is stained with a six-color immunophenotyping panel consisting of anti-mouse direct conjugates: (A) CD3e Alexa Fluor® 647; (B) CD19 PE-Cy®5.5; (C) CD4 PE-Cy®7, CD8 Alexa Fluor®488, CD11c APC-Cy®7, and NK1.1 PE direct antibody conjugates.


The Blue/Violet Configuration
This configuration is best suited for use with Molecular Probes® violet laser–excitable reagents to achieve optimal results in many applications, including:

• Cell proliferation
• Marine sample analysis
• Stem cell side population
• Live cell cycle analysis

In addition, the blue/violet configuration delivers optimal results using the no-lyse, no-wash protocol (Figure 2).



Figure 2. Mouse immunophenotyping using the no-lyse, no-wash method eliminates cell loss. Density plot analysis of 5-color panel. (A) Daughter plot of CD45+ gate showing the B cells stained with rat anti–mouse CD45R Pacific Blue™ conjugate (parameter VL1-H, violet laser, filter 450/40 nm BP) and T cells stained with hamster anti–mouse CD3 PE-Cy®5 (parameter BL3-H, blue laser, filter 530/30 nm BP). (B) A second density dot plot, a daughter of the CD3– CD45R– gate, showing the double-positive granulocytes stained with rat anti–mouse CD11b PE (parameter BL2-H, blue laser, 575/24 nm BP filter) and rat anti–mouse GR-1 Pacific Orange™ conjugate (parameter VL3-H, violet laser, 603/48 nm BP filter). The CD11b+ GR1– population represents phagocytes (monocytes, macrophages, and any circulating dendritic cells).

Applications for the Attune® Cytometer

With the Attune® Acoustic Focusing Cytometer, you can perform many standard and specialized research applications, including:

Immunophenotyping Cell cycle analysis Cell proliferation assays Apoptosis Live/dead cell discrimination

Rare-event detection Detection of phosphoproteins and phagocytosis Marine sample analysis Microbiological applications Other intracellular markers