MicroSEQ® Food Pathogen Detection Solution

A Complete, Easy-to-Use Solution for Detecting E. coli O157:H7, Listeria monocytogenes, Listeria spp., and Salmonella spp.

Our fully integrated, real-time PCR-based MicroSEQ® Pathogen Detection Solution combines sample preparation kits, lyophilized pathogen detection assays, instruments, and data analysis software.

This turnkey system is designed for ease of use, so you can deploy it rapidly in your food testing laboratory with minimal training. To enable you to detect E. coli O157:H7, Listeria monocytogenes, Listeria spp., and Salmonella spp. as quickly, easily, and reliably as possible, we have performance-verified all components of the system.

System Features Include:

Easy-to-use, streamlined lyophilized format
Faster chemistry, resulting in shorter time to results
High sensitivity and specificity for confidence in results
Optimal performance for a wide range of food sample types
Internal positive control helps eliminate false negatives
Assay design is based on existing TaqMan® kits

Products
Publications & Literature

Experimental Workflow for MicroSEQ® Food Pathogen Detection

Step 1:
Enrich Sample
Step 2:
Prepare DNA
Step 3:
Run Test
Step 4:
View Results

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Step 2: Prepare DNA

Extract DNA from your sample and prepare it for real-time PCR analysis.

Products for Step 2: Prepare DNA

Reagents & Kits

PrepSEQ™ Rapid Spin Sample Preparation Kit: Use the PrepSEQ™ Rapid Spin Sample Preparation Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples.

PrepSEQ™ Nucleic Acid Extraction Kit: The PrepSEQ™ Nucleic Acid Extraction Kit produces high-quality bacterial DNA samples for PCR-based detection from a wide range of food and environmental samples. These samples can be challenging to process because they often contain PCR inhibitors. For laboratories looking to achieve better real-time PCR results and improve overall efficiency, the PrepSEQ™ Nucleic Acid Extraction Kit meets these needs.

Instruments & Accessories

MagMAX™ Express-96 Magnetic Particle Processors: MagMAX™ Express magnetic particle processors are ideal for researchers who want to automate their MagMAX sample preparation kits. The MagMAX Express platform is the premier automated platform to meet your throughput needs.

Step 3: Run Test

Amplify DNA targets using a MicroSEQ® Pathogen Detection Kit and the 7500 Fast Real-Time PCR System.

Products for Step 3: Run Test

MicroSEQ® Detection Kits

MicroSEQ® E. coli O157:H7 Detection Kit

The MicroSEQ® E. coli O157:H7 Detection Kit detects E. coli O157:H7 simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Validated by the AOAC Research Institute
Time to results in as little as 8 hours
99% specificity to reduce false positive results
Detects 10³ cfu/mL enrichment solution

MicroSEQ® Listeria monocytogenes Detection Kit

The MicroSEQ® Listeria monocytogenes Detection Kit detects Listeria monocytogenes simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Validated by the AOAC Research Institute
27 hour total time to result
99% specificity to reduce false positive results
Detects 10⁴ cfu/mL enrichment solution

MicroSEQ® Listeria spp. Detection Kit

The MicroSEQ® Listeria spp. Detection Kit detects Listeria simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism-specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

27-31 hour total time to result
99% specificity to reduce false positive results
Effective for both food and environmental sample types

MicroSEQ® Salmonella spp. Detection Kit

The MicroSEQ® Salmonella spp. Detection Kit detects Salmonella spp. simply, reliably, and rapidly in food samples. The assay uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence.

Validated by the AOAC Research Institute
18 hour total time to result
99% specificity to reduce false positive results
Detects 10³ cfu/mL enrichment solution

Instruments & Accessories

7500 Fast Real-Time PCR System: The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.

Applied Biosystems ™ – by Life Technologies™

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