Human Identification
Consider the Evidence™
The Most Trusted, Integrated Human Identification SolutionsPrecise results. Consistent performance. Proven success, case after case. It's what you expect from Applied Biosystems. As the worldwide leader in DNA typing solutions, we deliver the most comprehensively validated products, expertise and application support available to human identification laboratories. From our trusted AmpFℓSTR® kits to industry-leading, high-performance genetic analyzers and software, to in-depth training and on-site technical assistance, it all works together to assure your success and maximize your productivity.
Training
Theory-based and hands-on laboratory sessions give you in-depth instruction on our full suite of human identification solutions.
Validation
Learn more about the most comprehensively validated products available for STR-based human identification. Order Validation and Verification Atlas CDs.
Customer Support
Contact technical specialists to answer your questions, review data, and troubleshoot results. Download articles and other support resources.
Automation
HID EVOlution™ Systems reduce hands-on time, minimize pipetting errors, and streamline the transfer of sample data between Tecan and Applied Biosystems instruments.
Experimental Workflow: Find the Products You Need for Every Step
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Step 1:
Sample Collection -
Step 2:
Sample Preparation -
Step 3:
DNA Quantification -
Step 4:
PCR Amplification -
Step 5:
Genetic Analysis -
Step 6:
Data Interpretation
Click the arrows above to view products
Step 1: Sample Collection
The Buccal DNA Collector™, manufactured by Bode Technology and distributed by Life Technologies, is an easy-to-use non-invasive device designed for collection of cheek buccal cells for human DNA identification analysis. Life Technologies is now a distributor for the Buccal DNA Collector™ worldwide (except in the United States).
Products for Step 1: Sample Collection
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Buccal DNA Collector™
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The Buccal DNA Collector™, manufactured by Bode Technology and distributed by Life Technologies, is an easy-to-use non-invasive device designed for collection of cheek buccal cells for human DNA identification analysis.
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4N6FLOQSwabs™, Regular Tip
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4N6FLOQSwabs™, Regular Tip are comprised of a solid, molded plastic applicator shaft with a regular-sized tip that can vary in size and shape.
Step 2: Sample Preparation
The PrepFiler™ kit uses uniquely structured magnetic particles with a breakthrough, multi-component surface chemistry to improve the quantity and quality of DNA isolated from forensic samples. Designed with a streamlined, easy-to-automate workflow, the robust new kit enables inhibitor removal and helps improve the overall yield, concentration and purity of DNA isolated from both routine and challenging samples. This provides greater quantities of highly purified DNA for improved downstream success.
Products for Step 2: Sample Preparation
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AutoMate Express™ Forensic DNA Extraction System
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The AutoMate Express™ Forensic DNA Extraction System is an easy to use, robust bench-top instrument that utilizes the PrepFiler Express™ and PrepFiler Express BTA™ chemistries packaged in pre-filled, foil sealed cartridges.

Sample Preparation (DNA Extraction) Kits
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PrepFiler™ Forensic DNA Extraction Kit (Updated)
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The PrepFiler™ Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior yield from forensic samples.

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PrepFiler™ Forensic DNA Extraction Kit
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The PrepFiler™ Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior yield from forensic samples.
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PrepFiler™ Automated Forensic DNA Extraction Kit (Updated)
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The PrepFiler™ Automated Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior yield from forensic samples and is designed to adapt easily to automation on various liquid handling platforms.
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PrepFiler™ Automated Forensic DNA Extraction Kit
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The PrepFiler™ Automated Forensic DNA Extraction Kit uses magnetic particles with an optimized multi-component surface chemistry to deliver superior yield from forensic samples and is designed to adapt easily to automation on various liquid handling platforms.
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PrepFiler™ BTA Forensic DNA Extraction Kit
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The PrepFiler™ BTA Forensic DNA Extraction Kit has been developed for the extraction of DNA from calcified tissues (bone, tooth), as well as certain adhesive-containing samples.
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Prep-n-Go™ Buffer
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The Prep-n-Go™ Buffer is designed to enable high quality direct PCR amplification of non-FTA® reference samples without the need for heat incubation, extraction, or purification.
Validated HID Automated System
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HID EVOlution™
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This system combines the PrepFiler™ Automated Forensic DNA Extraction Kit and the Tecan Freedom EVO® 150 automated liquid-handling workstation to provide a complete, validated solution for robust, reliable automation of forensic DNA extraction.
Step 3: DNA Quantification
Quantification of human DNA samples is essential for optimal STR performance. Our Quantifiler® kits provide everything you need to quickly and precisely quantitate human DNA. Validated as part of an integrated system, these kits use proven TaqMan® real-time PCR technology to specifically detect human and/or male DNA in samples that may vary widely in terms of DNA source, quality and concentration. The kits detect the broadest range of DNA concentrations (greater than 2,000-fold dynamic range) using a simple protocol that requires minimal hands-on time and results in more consistent STR amplifications.
Products for Step 3: DNA Quantification
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Quantifiler® Duo DNA Quantification Kit
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The Quantifiler® Duo kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction.
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Quantifiler® Human DNA Quantification Kit
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Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources.
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Quantifiler® Y Human Male DNA Quantification Kit
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The first commercially available kit for male DNA quantification, a valuable tool for sexual assault sample analysis.
Instruments & Accessories
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7500 Real-Time PCR System for Human Identification
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An advanced, validated real-time system for casework, databasing, and paternity applications.
Software
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HID Real-Time PCR Analysis Software v1.1
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Automatically assesses DNA sample quality and quantity to help you select the optimal AmpFℓSTR® Kit and the amount of DNA for STR analysis. It also provides a streamlined quantitation workflow, enhanced data review, and the ability to improve STR analysis efficiency.
Step 4: PCR Amplification
AmpFℓSTR® kits combine the sensitivity of PCR chemistry with the power of multicolor fluorescence technology to deliver robust and reliable results for all Human Identification applications—even with mixed, degraded, and challenged samples. The majority of the worldwide DNA casework and database laboratories have been built using AmpFℓSTR® kits.
For more information on AmpFℓSTR® kits, including amplified loci, dye labels, and probabilities of identity, view our AmpFℓSTR® Kit Product Portfolio (PDF).
Products for Step 4: PCR Amplification
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AmpFℓSTR® NGM™ PCR Amplification Kit
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The NGM™ Kit is a highly robust STR multiplex kit which utilizes a single protocol to amplify the 10 SGM Plus™ loci together with the additional loci approved by the European Union Council for the expansion of the European Standard Set.
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AmpFℓSTR® NGM SElect™ PCR Amplification Kit
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The NGM SElect™ Kit is a highly robust, single amplification STR multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect™ Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization.
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AmpFℓSTR® NGM SElect™ Express Kit
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The AmpFℓSTR® NGM SElect™ Express Kit enables extremely efficient analysis of single source samples for laboratories wishing to utilize the European Standard Set Loci (ESSL) together with additional loci including the highly discriminating SE33 locus.
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AmpFℓSTR® Identifiler PCR Amplification Kit
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The single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems. The Identifiler® kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards.
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AmpFℓSTR® Identifiler Plus PCR Amplification Kit
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Optimized for casework, the Identifiler® Plus kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence.
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AmpFℓSTR® Identifiler Direct PCR Amplification Kit
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Enables you to amplify the 16 loci included in the Identifiler® Kit from single source samples spotted on FTA® Cards, without the need to perform any DNA extraction or purification. Blood or buccal samples on FTA® Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without any reduction in data quality.
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AmpFℓSTR® YFiler PCR Amplification Kit
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Enables the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.
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AmpFℓSTR® MiniFiler PCR Amplification Kit
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As the world's first commercially available miniSTR kit, the AmpFℓSTR® MiniFiler™ PCR Amplification Kit increases your ability to obtain DNA results from compromised samples that previously would have yielded limited or no genetic data. This means cold cases can come off the shelf for re-analysis and new, challenging samples have a better chance of delivering interpretable results
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AmpFℓSTR® SGM PLUS® Kit
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Amplifies 10 STR loci and the gender marker Amelogenin yielding a highly informative kit for human identification, with a 1 in 5 X 10¹² average probability of identity.
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AmpFℓSTR® Profiler Plus ID PCR Amplification Kit
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Amplifies nine tetranucleotide short tandem repeat (STR) loci and the Amelogenin locus in a single reaction tube. The STR loci amplified are D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and vWA.
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AmpFℓSTR® Profiler PCR Amplification Kit
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Amplifies nine short tandem repeat (STR) loci and the Amelogenin locus in a single PCR amplification. The STR loci amplified are CSF1PO, D3S1358, D5S818, D7S820, D13S317, FGA, TH01, TP0X, and vWA.
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AmpFℓSTR® COfiler® Kit
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Complements the existing AmpFℓSTR® Profiler Plus® PCR Amplification Kit. The two kits provide human identification laboratories with the ability to generate information for all 13 core STR loci required by the Combined DNA Index System (CODIS) database in just two PCR amplifications.
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AmpFℓSTR® SEfiler Plus™ PCR Amplification Kit
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Uses a high performance PCR buffer system and re-optimized PCR conditions for improved "first pass" success rate with challenging samples. SEfiler Plus amplifies 12 total markers, including the eight STR loci required by the German DNA database, and has been designed to be a single solution for laboratories that process casework or high volume DNA database samples.
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AmpFℓSTR® SEfiler™ PCR Amplification Kit
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Amplifies 11 tetranucleotide repeat loci, including the SE33 locus, and the gender-determining locus Amelogenin.
Validated Instrument
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96-Well GeneAmp® PCR System 9700
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GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules.
Step 5: Genetic Analysis
Applied Biosystems capillary electrophoresis instrument systems have been validated by the Human Identification community and are the most widely trusted systems available.
Products for Step 5: Genetic Analysis
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3500 Genetic Analyzer for Human Identification
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A new standard in capillary electrophoresis with the 8-capillary 3500 Genetic Analyzer. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application.
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3500xL Genetic Analyzer for Human Identification
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A new standard in capillary electrophoresis with the 24-capillary 3500xL Genetic Analyzer. The 3500-series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application.
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3130xl Genetic Analyzer
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Offering 16-capillary throughput, advanced automation capabilities and superior performance, this system is ideal for laboratories that need streamlined set-up and 24-hour unattended operation.
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3100 Genetic Analyzer
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A multi-color fluorescence-based DNA analysis system with 16 capillaries operating in parallel, offers high-quality data and efficient sample processing.
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3130 Genetic Analyzer
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The latest generation of 4-capillary electrophoresis instrument offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity. Upgradeable to 16 capillaries whenever you are ready.
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3100-Avant Genetic Analyzer
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Upgradeable, 4-capillary ABI PRISM® 3100-Avant Genetic Analyzer gives you the sophisticated automation and superior performance of the 3100 system, with acquisition and operating costs tailored to a growing laboratory.
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310 Genetic Analyzer
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Affordable, versatile single-capillary analyzer.
Step 6: Data Interpretation
GeneMapper® ID Software has undergone extensive testing to ensure robust performance, reproducibility, and ease of use. Novel Process Component-Based Quality Values (PQVs) allow you to quickly identify and sort genotypes based on user-defined quality thresholds and automatically analyze multiple samples from different AmpFℓSTR kits. CODIS compatible reporting simplifies data uploading and increases lab productivity.
Products for Step 6: Data Interpretation
Software
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GeneMapper® ID-X Software
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gA powerful data analysis tool that significantly reduces the amount of time spent on routine data analysis and manual review. This system integrates novel and enhanced expert system functionality with improved manual review features to provide forensic scientists with a single, easy-to-use tool that improves lab productivity.
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GeneMapper® ID Software v3.2
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Combines the precision-sizing capabilities of GeneScan® software with the allele-calling power of Genotyper® software.
- Yfiler™ Haplotype Database
- Y-STR Haplotype Reference Database (YHRD)
- US Y-STR Database
- NIST STRBase
- Forensic News Past Issues
- DNAResource.com
- California Department of Justice
- Department of Pathology and Anatomy, University of North Texas
- Federal Bureau of Investigation
- NIJ Missing Persons Initiative & Links
- National Forensic Science Technology Center (NFSTC)
- Considerations for Efficient Implementation of the 3500 Series Genetic Analyzer for Human Identification Webinar Series
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Development of the AmpFℓSTR® NGM SElect™ Kit: New Sequence Discoveries and Implications for
Genotype Concordance - Next Generation STR Kits: A New Generation of Interpretation and Implementation Considerations
- Development of the AmpFℓSTR® NGM™ PCR Amplification Kit
- Extraction of High Quality Genomic DNA from Forensic Samples
- 2009 North America Future Trends in DNA Technology Seminar Series Presentations
- 2008 America Future Trends in DNA Technology Seminar Series Presentations
- The Impact of miniSTR Technology on Forensic Casework
- Developmental Validation of the AmpFℓSTR® MiniFiler™ PCR Amplification Kit
- Enhanced Quantifiler® Kit Data Analysis using HID Real-Time PCR Analysis Software
- Troubleshooting - Maximizing Quantifiler® Kit Data Quality
- GeneMapper® ID-X Software v1.1 Development of an Automated Approach for the Analysis and Interpretation of DNA Mixtures
What's New
- AmpFℓSTR® Identifiler® Plus PCR Amplification Kit
- AmpFℓSTR® Identifiler® Direct PCR Amplification Kit
- 3500 Series Genetic Analyzers
- AmpFℓSTR® NGM™ and NGM SElect™ Kits
- PrepFiler® Forensic DNA Extraction Kit
- AmpFℓSTR® MiniFiler™ PCR Amplification Kit
- GeneMapper® ID-X Software
- HID EVOlution® Systems
- Quantifiler® Duo DNA Quantification Kit
- Validation Support & Training Program
- VALID™ Software
Training
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