Step 1: Isolate Viral RNA

Products for Step 1: Isolate Viral RNA

MagMAX™ Express Magnetic Particle Processor

MagMAX™ Express magnetic particle processors are ideal for researchers who want to automate their MagMAX sample preparation kits. The MagMAX Express platform is the premier automated platform to meet your throughput needs. It seamlessly incorporates the rapid, reliable, and cost efficient magnetic bead-based extraction of nucleic acids that you expect from MagMAX technology.

MagMAX™ Express-96 Magnetic Particle Processor

MagMAX™ Express magnetic particle processors are ideal for researchers who want to automate their MagMAX sample preparation kits. The MagMAX Express platform is the premier automated platform to meet your throughput needs. It seamlessly incorporates the rapid, reliable, and cost efficient magnetic bead-based extraction of nucleic acids that you expect from MagMAX technology.

MagMAX™ Viral RNA Isolation Kits

Obtain high yields of viral RNA from biological fluids and cell-free samples such as respiratory swabs, using magnetic bead-based separation technology. These are available in single-tube and 96-well formats.

Invitrogen Products

View more reagents, kits and bench-top devices on invitrogen.com

iPrep™ Purification Instrument
The iPrep™ Purification Instrument is a closed system benchtop instrument that offers a wide portfolio of kits for fully automated nucleic acid purification. It processes up to 13 samples in less than 30 minutes, enabling sample integrity with pre-filled, sealed reagent cartridges. Just load the cartridge, add samples and select a protocol.

iPrep™ PureLink™ Virus Kit
The iPrep™ PureLink™ Virus Kit Kit allows for a simple yet standardized procedure for isolating viral RNA from a range of cell-free samples, using bead-based technology.

iPrep™ PureLink™ Viral RNA/DNA Mini Kit
Isolates high-quality and highly concentrated viral DNA and RNA from cell-free samples such as serum, plasma, and cell culture –ideal for downstream applications such as real-time PCR. Available in both spin-column and 96-well plate formats for fast and easy isolation.

Step 2: Reverse Transcribe & Amplify cDNA

Products for Step 2: Reverse Transcribe & Amplify cDNA

Invitrogen Products

View more reagents, kits and bench-top devices on invitrogen.com

SuperScript® III Platinum® One-Step qRT-PCR Kit
This kit combines powerful reverse transcriptase and DNA polymerase enzymes to convert and amplify influenza sequences in a single, convenient one-step protocol.

Step 3: Detect Influenza Subtype

Products for Step 3: Detect Influenza Subtype

	Pandemic H1N1/09 Assay Set v 2.0 This product employs assay designs licensed from the Centers for Disease Control and Prevention (CDC). Includes the primers and probes required to run the CDC Influenza 2009 A(H1N1) pdm Real-time rRT-PCR Detection Protocol. Catalog No. 4456927- not available for sale in the US or Japan
	Pandemic H1N1/09 Assay Controls v 2.0 Includes 5 controls (4 positive controls (1 for each assay) and 1 negative template control). Catalog No. 4456926- not available for sale in the US and Japan

Step 4: Perform Real-Time PCR

Products for Step 4: Perform Real-Time PCR

7500 Fast Real-Time PCR System

Primer and probe sets developed for the amplification of influenza subtypes may be detected on the Research Use Only Applied Biosystems® 7500 Fast Real-Time PCR System. The 7500 Fast System is a 96-well, 5-color real-time PCR system that is fully optimized for fast cycling. The 7500 Fast System delivers high-quality results in as little as 30 minutes, and is compatible with both 8-tube strips and 96-well plates to accommodate varying sample throughputs.

7500 Fast Service Contract

Plates, Tubes & Plastics

MicroAmp® Fast 8-Tube Strip, 0.1 mL

The MicroAmp® Fast 8-Tube Strip fits snugly in the Veriti® 96-Well Thermal Cycler, enabling maximum thermal conductivity and providing precise control of cycling temperatures.

MicroAmp® Optical 8-Cap Strip

MicroAmp® Caps Strips are designed to fit on MicroAmp® Reaction Tubes, reaction strips and 96-well plates. They are available in 8-cap and 12-cap strips in clear and colored sets of red, orange, blue, and green. MicroAmp® Optical 8-Cap Strip should be used for Real-Time PCR applications.

MicroAmp® Fast Optical 96-Well Reaction Plate with Barcode, 0.1 ml

The MicroAmp® Fast Optical 96-Well Reaction Plate is an integral component of the Applied Biosystems 7500 and 7900HT Fast Real-Time PCR Systems.