Thermal Cyclers

A Better Than Gradient Approach to Thermal Cycling

1. PCR Optimization: each block can be set with a specific temperature which is ideal for precise control over PCR optimization.
2. Run More Experiments: the six peltier blocks can also be used to run up to six different annealing temperatures in the same run. With this enhanced functionality,the Veriti 96-well Thermal Cycler with VeriFlex Blocks is the ideal thermal cycler for any lab.

• Use the on-board Tm calculator to approximate the optimal annealing temperature.
• Run up to six separate temperatures in the same plate to determine the optimal annealing temperature.
• VeriFlex Blocks maintain their thermal characteristics between optimizing and isothermal conditions—eliminating the need for further optimization steps.

VeriFlex Blocks: More than just for PCR optimization
VeriFlex Blocks have an additional benefit over gradient thermal cyclers. Since the peltier blocks are separate, it is possible to run up to six different temperatures in the same protocol step (Figure 1).

• Run up to six different annealing temperatures for cycle sequencing or endpoint PCR.
• Incubate samples at six different temperatures simultaneously.

	Figure 1A. Screenshot from Veriti® 96-well Thermal Cycler showing the annealing temperature being set for six different primers.
	Figure 1B. VeriFlex™ Blocks: Six individual peltier blocks.
	Figure 1C. PCR results showing six primer sets run in a single PCR amplification cycle. Results indicate that the Veriti 96-well Thermal Cycler can run six assays at six annealing temperatures during the same PCR run.