Real-Time PCR
Real-Time PCR—also called quantitative polymerase chain reaction (qPCR)—is one of the most powerful and sensitive gene analysis techniques available and is used for a broad range of applications including quantitative gene expression analysis, SNP genotyping, copy number analysis, pathogen detection, drug target validation and for measuring RNA interference. Frequently, real-time polymerase chain reaction is combined with reverse transcription to quantify messenger RNA (mRNA) and microRNA (miRNA) in cells or tissues.
New to Real-Time PCR? Start Here!
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Real-Time PCR InstrumentsWe have a wide range of medium- to high-throughput instruments for every application and budget. |
TaqMan® Assays & ReagentsTaqMan® Probe-Based products for gene expression, protein, miRNA, ncRNA, SNP, and CNV analysis. |
High Resolution Melting ProductsIdentify variations in nucleic acid sequences using this new post-PCR analysis method. |
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Application Guides—Using Real-Time PCR in Your Research
miRNA & Non-Coding RNA Analysis
Methylation Analysis
Detect methylation differences between genes using real-time PCR-based high resolution melting (HRM) analysis.
Influenza A (H1N1) Virus Detection
Reagents and systems for RNA isolation, reverse transcription, amplification, and assay detection.
Stem Cell Research
Solutions for analyzing stem cells, determining stemness, and for studying gene regulation and translation in stem cells.
Protein Expression
TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of protein markers from limited quantities of cultured cells.
Food Pathogen Detection
Detect multiple bacteria in the same run, including Salmonella, Campylobacter, E. coli O157:H7, and Listeria monocytogenes.
Pharmaceutical Analytics
Detect Mycoplasmas, viruses, and residual host cell contamination during pharmaceutical, cosmetics, and personal care product manufacturing.
