Copy Number Variation

Step 1: Prepare Sample

Products for Step 1: Prepare Sample

DNA Isolation Kits

Kits for isolating DNA from blood, cells, tissues, plasmids, and more.

Step 2: Select Assays

Products for Step 2: Select Assays

Pre-designed and Custom Plus TaqMan® Copy Number Assays

TaqMan® Copy Number Assays are designed for analysis of copy number variations (CNVs) and smaller regions in human and mouse genomes. The human pre-designed assay collection includes over 1.6 million assays with genome-wide coverage. The mouse collection includes over 180,000 assays targeting gene exons. Assays to common vector marker and reporter genes are also available for transgenic studies. Custom Plus assays undergo thorough bioinformatic analysis and have the quality of pre-designed TaqMan® Copy Number Assays but are available for user-defined human and mouse genomic regions of interest.

Custom TaqMan® Copy Number Assays

Custom TaqMan® Copy Number Assays combine TaqMan® Assay Chemistry with Applied Biosystems real-time PCR instruments to enable the generation of specific, reproducible and easy-to-interpret copy number results. Ideally suited for validation studies and large sample screens, the Custom TaqMan® Copy Number Assays provide a flexible targeted approach to copy number analysis.

TaqMan® Copy Number Reference Assays

TaqMan® Copy Number Reference Assays are run with pre-designed, Custom Plus, and Custom TaqMan® Copy Number Assays in a duplex real-time PCR reaction to detect and measure copy number variations in the human and mouse genomes.

Step 3: Amplify DNA

Products for Step 3: Amplify DNA

Reagents & Kits

TaqMan® Genotyping Master Mix

Obtain unrivaled cluster resolution for unambiguous SNP allelic discrimination. Optimized for end-point fluorescence detection in genotyping applications.

Instruments & Accessories

StepOnePlus™ Real-Time PCR System

Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

7500 Fast Real-Time PCR System

The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.

7500 Real-Time PCR System

The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

7300 Real-Time PCR System

Get the Applied Biosystems advantage at an affordable price; the 7300 Real-Time PCR System balances exceptional quality with budget sensitivity.  This 3rd-generation system is built on over 10 years of real-time expertise and is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications with the highest confidence in results.

7900HT Fast Real-Time PCR System

The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

ViiA™ 7 Real-Time PCR System

The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.

Step 4: Analyze Data

Products for Step 4: Analyze Data

Software

CopyCaller™ Software

CopyCaller™ Software enables you to perform relative quantitation analysis of genomic DNA targets using the real-time PCR data from pre-designed, Custom Plus, or Custom TaqMan® Copy Number Assay experiments. The software and associated copy number assays can be used to detect and measure copy number variation of specific sequences in the human and mouse genomes.