Copy Number Variation
TaqMan® Copy Number Assays combine TaqMan® MGB probe chemistry with Applied Biosystems real-time PCR instruments to form a method for obtaining specific, reproducible, and easy-to-interpret copy number results. This method is fast and simple, and can be completed in hours rather than days. TaqMan® Assays are the gold standard for accurate target quantitation, making them ideal for use in microarray follow-up studies. TaqMan® Copy Number Assays can be used to screen specific targets, and the workflow can be automated so several hundred to thousands of samples can be processed in a single day
The TaqMan® Copy Number Assay Product Family includes the following:
- Pre-designed TaqMan® Copy Number Assays
- Human: over 1.6 million pre-designed assays available for genome-wide coverage
- Mouse: over 180,000 pre-designed assays available for gene exons
- Common vector marker and reporter genes assays
- Custom Plus TaqMan® Copy Number Assays for user-defined human and mouse genomic targets
- Custom TaqMan® Copy Number Assays for other targets of interest
- Submit masked target sequences for assay design
- Submit primer/probe pair sequences for assay synthesis
As the simplest method available to study copy number variation, the TaqMan® Copy Number Assays feature easy assay ordering and a simple workflow to enable quick results.
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Products
Experimental Workflow: Find the Products You Need for Every Step
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Publications & Literature
Product Bulletins
- TaqMan® Copy Number Assays Includes information on pre-designed, Custom Plus, and Custom TaqMan® Copy Number Assays, and TaqMan® Copy Number Reference Assays.
Posters
- Determining the Copy Number of Genes Using Real-Time Quantitative PCR
- Selecting SNPs for Genetic Association Studies Based on the Genome-wide Patterns of Linkage Disequilibrium Inferred from the HapMap Project Data
Application Notes
Background References
- Copy Number Variants and Common Disorders: Filling the Gaps and Exploring Complexity in Genome-Wide Association Studies Publication: PLoS Genet 2007; 3(10): e190. doi:10.1371/journal.pgen.0030190
- Detection of large-scale variation in the human genome Publication: Nature Genetics 2004; 36, 949 - 951
- Large-Scale Copy Number Polymorphism in the Human Genome Publication: Science 23 July 2004: Vol. 305. no. 5683, pp. 525 - 528
- Global variation in copy number in the human genome Publication: Nature 444, 444-454 (23 November 2006)
Citations
- Differential quantification of CYP2D6 gene copy number by four quantitative real-time PCR assays Publication: Pharmacogenetics and Genomics 2010, 20:451-454.
- Recurrent microdeletions at 15q11.2 and 16p13.11 predispose to idiopathic generalized epilepsies Publication: Brain. 2010; 133:23-32; doi:10.1093/brain/awp262
- Copy Number Variants of GSTM1 and GSTT1 in relation to lung cancer risk in a prospective cohort study. Publication: Ann Epidemiol. 2009; 19(8):546-52
- Sample degradation leads to false-positive copy number variation calls in multiplex real-time polymerase chain reaction assays Publication: Analytical Biochemistry. 2009 Mar 15;386(2):288-90.
- Origins and functional impact of copy number variation in the human genome Publication: Nature advance online publication 7 October 2009 | doi:10.1038/nature08516; Received 14 August 2009; Accepted 21 September 2009; Published online 7 October 2009
- A recurrent 15q13.3 microdeletion syndrome associated with mental retardation and seizures. Publication: Nature Genetics. 2008 Mar;40(3):322-8.
- Recurrent rearrangements of chromosome 1q21.1 and variable pediatric phenotypes. Publication: The New England Journal of Medicine. 2008 Oct 16;359(16):1685-99.
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