FAST Product Guide
Fast Is More Than You Know. Superior Performance in Half the Time.
- Fast-enabled instrumentation: specially designed Fast block helps ensure thermal uniformity at top speeds
- TaqMan® Fast Advanced Master Mix, TaqMan® Fast Virus 1-Step Master Mix, Fast SYBR® Green and Express qPCR SuperMix and 1-Step kits enable rapid results with excellent performance equivalent to or better than that of standard real-time PCR approaches
- Fast optical plates ensure excellent precision in 10–30 µL reaction volumes
- Fast ramp rates enable rapid results without compromising extension times or assay quality
| Instruments | ||||
|---|---|---|---|---|
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| Instrument/ Capacity | StepOnePlus™ 96-well plates, 0.1 mL tubes StepOne™ 48-well plates, 0.1 mL tubes | 7500 Fast 7500 Fast Dx 96-well optical Fast plates. | 7900HT 96 and 384 wells, 384-well microfluidic card | ViiA™ 7 96-well 384-well TaqMan® Array Micro Fluidic Cards |
| Temperature Uniformity | ± 0.5C within 30 seconds of clock start | ± 0.5C within 30 seconds of clock start | ± 0.5C within 30 seconds of clock start | ±0.5°C, within 30 sec of reaching temperature set point |
| Fast block |
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| Optional |
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Optics | ||||
| Excitation source | Blue LED | Tungsten-Halogen | 488 nm ion argon laser | OptiFlex™ System with Halogen lamp |
| Filters/Colors | StepOnePlus™ 4 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™, NED/TAMRA and ROX™ dyes StepOne™ 3 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™ and ROX™ dyes | 5 filter sets calibrated for FAM™ /SYBR® Green, VIC®/JOE™, NED™ /TAMRA™ /Cy3®, ROX™ /TEXAS RED, Cy5® dyes | None, CCD acts as spectrograph with continuous detection 500 -650nm | 6 excitation filters (450–670 nm) 6 emission filters (500–720 nm) |
| Detector | Photodiodes | CCD | CCD | CCD |
Performance Specifications | ||||
| Demonstrated Sensitivity | 10 copies | 10 copies | 10 copies | Down to 1 copy |
| Dynamic Range | 9 logs of linear dynamic range | 5 logs guaranteed, 9 logs demonstrated | 5 logs guaranteed, 9 logs demonstrated | Up to 9 logs of linear dynamic range |
Instrument Specs | ||||
| Size (W x D x H) | W 23.6 cm D 51.2 cm H 42.7 cm | W 34 cm D 45 cm H 49 cm | w/ drawer open: W 72 cm D 84 cm H 64 cm | W 53.3 cm D 63.5 cm H 64.5 cm |
Other | ||||
| Multiplex Quantitation |
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| Supported Chemistry | SYBR® Green dye, TaqMan® probes. Capable of running other chemistries | 7500 Fast & 7500 Fast Dx TaqMan® Gene Expression & Quantitation Assays supported in Fast Mode; SYBR® Green dye, TaqMan® probes in other modes. Capable of running other chemistries
| TaqMan® Gene Expression & Quantitation Assays supported in Fast Mode. SYBR®, TaqMan probes in other modes. Capable of running other chemistries | TaqMan® Gene Expression & Genotyping Assays, and SYBR® Green. Capable of running other chemistries. |
