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Methylation Analysis

MS-HRM Standard Curves
MS-HRM Standard Curves With Uniform Resolution From 0% to 100% Methylation.

Methylation Analysis Using Methylation-Sensitive HRM and DNA Sequencing.


DNA methylation is a key epigenetic mechanism regulating gene expression and chromatin structure.

MS-HRM followed by Sanger-based DNA sequencing is a fast, simple method for methylation analysis that can measure methylation levels as low as 0.1–2%.

Procedure Overview
MS-HRM analysis offers a simple method for quickly analyzing the methylation status of specific genetic loci. Reaction products that merit further analysis can then be sequenced directly to identify precise methylation patterns.

Applied Biosystems provides an integrated set of tools for locus-specific DNA methylation analysis using an optimized, streamlined procedure.

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