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  9. Overview of HRM Products

Overview of HRM Products

The Applied Biosystems family of integrated HRM products - including instrumentation, software, and reagents - have been optically, thermally, and chemically optimized to provide the highest-resolution melt analysis available today.

Applied Biosystems HRM Products


Instrument Software PCR Reagents Calibration Controls

ViiA™ 7 Real-Time PCR System (384-well, 96-well)

The new ViiA™ 7 System is a 7th generation real-time PCR system that enables high-productivity qPCR through flexibility, performance, integrated TaqMan® Assay content, and the most advanced features available.


StepOne™ Real-Time PCR System (48-well) and StepOnePlus™ Real-Time PCR System (96-well)

The StepOne™ and StepOnePlus™ Systems are remarkably simple systems designed with a user-friendly, yet powerful, interface for researches of all experience levels.


7500 Fast Real-Time PCR System (96-well)

The 7500 Fast System offers maximum performance in minimum time. Fully optimized for Fast cycling, it delivers high-quality results in as little as 30 minutes.

ViiA™ 7 Software HRM Module
for ViiA™ 7 Real-Time PCR System and
HRM Software v3.0
for StepOne™, StepOnePlus™, and 7500 Fast Real-Time PCR Systems

The Applied Biosystems High Resolution Melt Software packages enable HRM analysis on our full line of gold-standard real-time PCR instruments. Each High Resolution Melt software package contains features that make fast and accurate HRM results more accessible than ever.


MeltDoctor™ HRM Master Mix

The MeltDoctor™ HRM Master Mix, developed and optimized solely for HRM applications, contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets.

MeltDoctor™ HRM Reagent Kit

The MeltDoctor™ HRM Reagent Kit provides all PCR components and the MeltDoctor™ HRM dye required for high resolution melting analysis.
MeltDoctor™ HRM Calibration Plates (96-Well Fast or 384-Well)

Ready-to-use MeltDoctor™ HRM Calibration Plates contain all the components required for pure dye and HRM calibration reducing the complexity of getting started with HRM experimentation. They provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves.

MeltDoctor™ HRM Calibration Standard

The MeltDoctor™ HRM Calibration Standard contains a DNA template and primers which, when mixed with PCR reagents and dye, can be used to prepare a plate for thermal and optical HRM calibration.
MeltDoctor™ HRM Positive Control Kit *

The MeltDoctor™ HRM Positive Control Kit provides components to demonstrate and troubleshoot HRM analysis. The kit consists of forward and reverse primers and three DNA templates representing alleles AA, AB and BB.

7900HT Fast Real-Time PCR System 384-well, 96-well)

The 7900HT Fast System combines 384-well plate compatibility with fully automated robotic loading enabling you to achieve unprecedented throughput and flexibility.

HRM Software v2.0
for 7900HT Fast Real-Time PCR System


High Resolution Melting (HRM) analysis is an alternative to dHPLC sequencing screening of new gene variants. The application does not require temperature shifting, which results in a greater likelihood of identifying new homozygous mutations than methods that require temperature shifting.