Long noncoding RNA (ncRNA) Analysis

Step 1: Prepare Sample

Products for Step 1: Prepare Sample

mirVana™ miRNA Isolation Kit

Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE

Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

TaqMan® MicroRNA Cells-to-CT™ Kit

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

TURBO DNA-free™ Kit

TURBO™ DNase is a recombinant, engineered form of DNase I that is more efficient than wild-type DNase I at digesting trace amounts of unwanted DNA in RNA samples. The TURBO DNA-free™ Kit comes with all reagents needed to perform 50 TURBO™ DNase treatments plus a highly effective DNase Inactivation Reagent, making this kit the tool of choice for RT-PCR sample preparation.

Step 2: Reverse Transcribe RNA

Products for Step 2: Reverse Transcribe RNA

High Capacity RNA-to-cDNA Kit

High Capacity RNA-to-cDNA Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.

Step 3: Amplify cDNA (optional)

Products for Step 3: Amplify cDNA (optional)

TaqMan® PreAmp Master Mix

TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and TaqMan® Gene Expression Master Mix.

Step 4: Set Up Non-coding RNA Assay Reaction

Products for Step 4: Set Up Non-coding RNA Assay Reaction

TaqMan® Gene Expression Master Mix

TaqMan® Gene Expression Master Mix is tailored for precise quantification by real-time PCR. It is designed for routine and challenging experiments.

TaqMan® Non-coding RNA Assays

TaqMan® Non-coding RNA Assays provide a highly reliable way to measure expression of long non-coding transcripts. These assays are based on the same gold standard TaqMan® Assays technology that is used in the TaqMan® Gene Expression Assays collection. With TaqMan® Assay technology, you get the high specificity and sensitivity you need for quantification and validation studies.

Step 5: Perform Real-Time PCR Amplification

Products for Step 5: Perform Real-Time PCR Amplification

StepOnePlus™ Real-Time PCR System

The Applied Biosystems® StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4-color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

7300 Real-Time PCR System

Get the Applied Biosystems advantage at an affordable price; the 7300 Real-Time PCR System balances exceptional quality with budget sensitivity.

7500 Real-Time PCR System

The Applied Biosystems® 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third-generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

7500 Fast Real-Time PCR System

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.

7900HT Fast Real-Time PCR System

The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading — and also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

Step 6: Analyze Data

Products for Step 6: Analyze Data

RealTime StatMiner® Software (optional)

RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.

DataAssist™ Software (optional)

DataAssist™ Software is an easy to use data analysis tool that utilizes the comparative Ct (ddCt) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.