There are a number of miRNA profiling techniques available, some requiring more steps and manual intervention than others. Using a 5' nuclease assay chemistry protocol is a fast and simple way to get data results. The experiment protocol involves combining purified genomic DNA, master mix, and a 5' nuclease assay, then thermal cycling, reading, and analyzing the results.
TaqMan® MicroRNA Assay 5' nuclease technology uses two allele-specific TaqMan® MGB probes and a PCR primer pair to detect the specific miRNA target. The probes and primers uniquely align with the genome, enabling TaqMan® Assay technology to provide unmatched specificity.
With your choice of using Applied Biosystems 384-well or greater than 384-well real-time PCR instrumentation, you can select the workflow that best fits your miRNA profiling project needs.
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For profiling studies across a small number of samples or limited sample material.
Delivers the high throughput capabilities required for large profiling studies.