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Pri-miRNA Analysis

Workflow Features

  • Ideal for measuring miRNA “gene” transcription in human, mouse or rat species
  • Enables the regulation of miRNA biogenesis to be probed when used in combination with TaqMan® MicroRNA Assays that detect mature miRNA sequences
  • Adds new perspective to your understanding of miRNA expression

How It Works
MicroRNA “genes” are transcribed in the nucleus creating a long pri-miRNA transcript. The pri-miRNA sequence contains one or more stem-loop sequences, each containing mature miRNA sequences. TaqMan® Pri-miRNA Assays are designed in close proximity to each independent stem-loop sequence identified in the Sanger miRBase sequence repository (http://microrna.sanger.ac.uk/sequences/) using Applied Biosystems’ state-of-the-art bioinformatics pipeline. This approach ensures that the primary transcript containing the stem-loop of interest is being measured.

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