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Real-Time PCR Instruments

Celebrating over 15 years of Innovation in Real-Time PCR


A leader in qPCR innovation, Applied Biosystems offers you the gold standard in real-time instrumentation. With our real-time PCR platforms you get the best value real-time PCR systems anywhere with excellent performance, reliability, and world-class support. Designed for compatibility with TaqMan® assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started and get the results you need.

StepOne Real-Time PCR Instrument 7500 Fast Real-Time PCR Instrument ViiA7 instrument QuantStudio Real-Time PCR Instrument
StepOnePlus™ Real-Time PCR System:
Easy to use
for all levels of experience 		Applied Biosystems® 7500 Real-Time PCR System:
Trusted performance and Dye-versatility 		ViiA™7 Real-Time PCR System:
Powerful Features and
Intuitive Design 		QuantStudio™ 12K Flex
Real-Time System::
Ultimate horsepower, flexibility and scability


 
StepOne/StepOnePlus™ Real-time PCR System
7500 Fast Real-time PCR System
7500 Real-time PCR System
ViiA™ 7 Real-Time PCR System
QuantStudio™ 12K Flex Real-Time System
Price / Varies
Speed
Colors 3/4 5 5 21 21
Ease of Use


Compare Specifications:

  • Instruments


    Instruments

    StepOne Real-Time PCR Instrument

    7500 Fast Real-Time PCR Instrument

    7900 HT Real-Time PCR Instrumeht

    ViiA

    QuantStudio Real-Time PCR Instrument

    Instrument/
    Capacity    		 StepOnePlus™
    96-well plates, 0.1 mL tubes

    StepOne™
    48-well plates, 0.1 mL tubes     		7500 Fast
    7500 Fast Dx
    96-well optical Fast plates.

    7500
    7500 for HID
    96 well optical plates or 0.2 mL tubes.    		 7900HT
    96 and 384 wells, 384-well microfluidic card    		 ViiA™ 7
    96-well
    384-well
    TaqMan® Array Micro Fluidic Cards
    		 QuantStudio™ 12K Flex System
    96-well standard (10–100 μL reactions)

    96-well Fast (10–30 μL reactions)

    384-well (5–20 μL reactions)

    TaqMan® Array Micro Fluidic Cards (~1 μL reactions)

    QuantStudio™ 12K Flex OpenArray™ Plates (~33 nL reactions)
    Temperature Uniformity ± 0.5C within 30 seconds of clock start ± 0.5C within 30 seconds of clock start ± 0.5C within 30 seconds of clock start ±0.5°C, within 30 sec of reaching temperature set point ± 0.25C
    Fast block Yes

    7500 Fast & 7500 Fast Dx
    Yes

    7500 & 7500 for HID
    No

     

    		 Optional Yes (96 well and 384 well) Yes
    (96 well)
    Optics
    Excitation source Blue LED Tungsten-Halogen 488 nm ion argon laser OptiFlex™ System with Halogen lamp Enhanced OptiFlex™ System-White LED
    Filters/Colors StepOnePlus™
    4 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™, NED/TAMRA and ROX™ dyes

    StepOne™
    3 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™ and ROX™ dyes    		 5 filter sets calibrated for FAM™ /SYBR® Green, VIC®/JOE™, NED™ /TAMRA™
    /Cy3®, ROX™ /TEXAS RED, Cy5® dyes    		 None, CCD acts as spectrograph with continuous detection 500 -650nm 6 excitation filters (450–670 nm)
    6 emission filters (500–720 nm)

    Decoupled—6 emission, 6 excitation

    6 excitation (450–670 nm)

    6 emission (500–720 nm)
    Detector Photodiodes CCD CCD CCD CCD
    Performance Specifications
    Demonstrated Sensitivity 10 copies 10 copies 10 copies Down to 1 copy Down to 1 copy
    Dynamic Range 9 logs of linear dynamic range 5 logs guaranteed, 9 logs demonstrated 5 logs guaranteed, 9 logs demonstrated Up to 9 logs of linear dynamic range Up to 9 logs of linear dynamic range

    Up to 7 logs of linear Dyanmic range (OpenArray) demonstrated
    Instrument Specs
    Size
    (W x D x H)    		 W 23.6 cm
    D 51.2 cm
    H 42.7 cm    		 W 34 cm
    D 45 cm
    H 49 cm    		 w/ drawer open:
    W 72 cm
    D 84 cm
    H 64 cm    		 W 53.3 cm
    D 63.5 cm
    H 64.5 cm    		 W 50.5 cm
    D 67.2 cm
    H 73.8 cm
    Other
    Multiplex Quantitation Yes Yes Yes Yes Yes (non-OpenArray)
    Supported Chemistry SYBR® Green dye, TaqMan® probes. Capable of running other chemistries 7500 Fast & 7500 Fast Dx
    TaqMan® Gene Expression & Quantitation Assays supported in Fast Mode; SYBR® Green dye, TaqMan® probes in other modes. Capable of running other chemistries

    7500 & 7500 for HID
    SYBR® Green dye, TaqMan® probes. Capable of running other chemistries

     

    		 TaqMan® Gene Expression & Quantitation Assays supported in Fast Mode. SYBR®, TaqMan probes in other modes. Capable of running other chemistries TaqMan® Assays, and SYBR® Green. Capable of running other chemistries. TaqMan® Assays, and SYBR® Green. Capable of running other chemistries for non-OpenArray® formats.
     

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