TaqMan® MicroRNA Profiling Using 384-Well Real-Time PCR Instruments
- Results the same day — complete an experiment profiling hundreds of miRNAs in as little as 5 hours
- Ideal for human, mouse, and rat profiling
- Requires only minute sample amounts — as little as 1 ng of total RNA input — making it suitable for FFPE, FACS, biopsy, and other very small samples
- Comprehensive coverage of known miRNAs consistent with Sanger miRBase v14
How it Works
Up to 381 miRNAs and controls are reverse transcribed in a single reaction using Megaplex™ RT Primers, a mixture of miRNA-specific stem-loop primers. Next, an optional amplification step can be performed using Megaplex™ PreAmp Primers. This unbiased amplification step significantly increases the concentration of miRNAs in the sample for maximum sensitivity and detection using real-time PCR. For the final quantitation step, TaqMan® Universal Master Mix II is added to each sample and the mixtures are pipetted into the sample loading ports of a TaqMan® Array MicroRNA Card—a pre-configured micro fluidic card containing 384 TaqMan® MicroRNA Assays. The real-time PCR is run on the Applied Biosystems® 7900HT Fast Real-Time PCR System.