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StepOne™ and StepOnePlus™ Real-Time PCR Systems


Instruments

StepOne Real-Time PCR Instrument

StepOne Real-Time PCR Instrument

Instrument/
Capacity		 StepOnePlus™
96-well plates, 0.1 mL tubes

StepOne™
48-well plates, 0.1 mL tubes
Temperature Uniformity ± 0.5C within 30 seconds of clock start ± 0.5C within 30 seconds of clock start
Fast block Yes Yes
Optics
Excitation source Blue LED Blue LED
Filters/Colors 4 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™, NED/TAMRA and ROX™ dyes 3 filters factory calibrated for FAM™/SYBR® Green, VIC®/JOE™ and ROX™ dyes
Detector Photodiodes Photodiodes
Performance Specifications
Demonstrated Sensitivity 10 copies 10 copies
Dynamic Range 9 logs of linear dynamic range 9 logs of linear dynamic range
Instrument Specs
Size
(W x D x H)		 W 23.6 cm
D 51.2 cm
H 42.7 cm		 W 23.6 cm
D 51.2 cm
H 42.7 cm
Other
Multiplex Quantitation Yes Yes
Supported Chemistry SYBR® Green dye, TaqMan® probes. Capable of running other chemistries SYBR® Green dye, TaqMan® probes. Capable of running other chemistries
 
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