There are a number of SNP analysis techniques available, some requiring more steps and manual intervention than others. Using a 5' nuclease assay chemistry protocol is a fast and simple way to get data results. The experiment protocol involves combining purified genomic DNA, master mix, and a 5' nuclease assay, then thermal cycling, reading, and analyzing the results.
TaqMan® SNP Genotyping Assay 5' nuclease technology uses two allele-specific TaqMan® MGB probes and a PCR primer pair to detect the specific SNP target. The probes and primers uniquely align with the genome, enabling TaqMan® genotyping technology to provide unmatched specificity.
With 4.5 million pre-designed TaqMan® SNP Assays available and a robust custom design pipeline, genotyping with TaqMan® Assays is an ideal method for validating and screening a low to medium number of SNP markers and a high number of samples. With a choice of Applied Biosystems 48-, 96-, 384 or greater than 384-well real-time PCR instrumentation, you can select the workflow that best fits your genotyping project needs.
Select Your Area of Interest:
For studying a small number of SNPs and fewer than 1,000 samples
Choose from over 2,700 unique assays that detect polymorphisms in all known high-value DME pharmacogenetics markers, including PharmaADME core markers.
For studying a low to medium number of SNPs and greater than 1,000 samples