SNP Genotyping Using 48-, 96-, and 384-Well Real-Time PCR Instruments
TaqMan® SNP Genotyping Assays provide optimized assays for genotyping SNPs. The products use 5´ nuclease assay chemistry for amplifying and detecting specific SNP alleles in purified genomic DNA samples. Each assay allows researchers to genotype individuals for a specific SNP.
- World’s largest collection of single-tube, ready to use SNP assays available
- Robust custom assay design pipeline provides enables highly accurate, reproducible and reliable results
- Easy ordering and a simple workflow enable quick results
- Suggested panels to get you started quickly for many applications
- Superior Applied Biosystems® Real-Time PCR Systems available to meet all throughput needs and budgets for processing and analyzing <1,000 samples a day
Experimental Workflow for SNP Genotyping
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Step 1:
Prepare Sample -
Step 2:
Select Assays -
Step 3:
Amplify DNA -
Step 4:
Allelic Discrimination -
Step 5:
Analyze Data
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Step 1: Prepare Sample
Isolate and purify genomic DNA.
Products for Step 1: Prepare Sample
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TaqMan® Sample-to-SNP™ Kit
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The TaqMan® Sample-to-SNP™ Kit provides a streamlined protocol for performing TaqMan chemistry-based genotyping analysis from any sample with a single kit.
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BloodPrep® DNA Chemistry for Cultured Cells and Blood
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BloodPrep® DNA Chemistry is designed to rapidly isolate DNA from fresh or frozen blood from any animal species, tissue-cultured cells, or buccal swabs. The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.
Step 2: Select Assays
Select or design TaqMan® probes.
Products for Step 2: Select Assays
SNP Genotyping Assays
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TaqMan® SNP Genotyping Assays
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With 4.5 million SNP assays available, including 3.5 million HapMap SNPs, 70,000 cSNPs and 160,000 validated assays, TaqMan® SNP Genotyping Assays make it easy to perform SNP genotyping studies with the precision of TaqMan® reagent-based chemistry.
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Custom TaqMan® SNP Genotyping Assays
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Order custom, single-tube TaqMan® reagent-based assays to perform genotyping studies with any possible SNP in any organism. Three different assay reaction sizes can be ordered to meet your project needs. The new Custom TaqMan® Assays Design Tool allows you to quickly order Custom TaqMan® Assays by submitting target sequences of interest or custom primer and probe sequences from any organism.
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TaqMan® Drug Metabolism Assays
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Genotype over 2,700 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes.
Step 3: Amplify DNA
Prepare reaction plate and amplify DNA using sequence specific primers and TaqMan® probe on an Applied Biosystems® Real-Time PCR System or thermal cycler.
Products for Step 3: Amplify DNA
Reagents & Kits
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TaqMan® Genotyping Master Mix
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Obtain unrivaled cluster resolution for unambiguous SNP allelic discrimination. Optimized for end-point fluorescence detection in genotyping applications.
Instruments & Accessories
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Veriti® 96-Well Thermal Cycler
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The Veriti® 96-Well Thermal Cycler delivers excellent PCR and the proven reliability of Applied Biosystems 9600 and 9700 GeneAmp® PCR instruments. With the added control of VeriFlex™ Blocks, you have six independent temperature blocks providing precise control over your PCR optimization.
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Dual Flat Block GeneAmp® PCR System 9700
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GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The System consists of a base module and one of many interchangeable sample block modules. The Dual Flat Block GeneAmp® PCR System 9700 is designed for use with TaqMan® OpenArray® Genotyping Plates on the OpenArray Real-Time PCR System.
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StepOnePlus™ Real-Time PCR System
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Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
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StepOne™ Real-Time PCR System
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Applied Biosystems’ 48-well low-throughput real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.
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7500 Fast Real-Time PCR System
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The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
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7500 Real-Time PCR System
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The 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
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7300 Real-Time PCR System
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Get the Applied Biosystems advantage at an affordable price; the 7300 Real-Time PCR System balances exceptional quality with budget sensitivity. This 3rd-generation system is built on over 10 years of real-time expertise and is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications with the highest confidence in results.
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7900HT Fast Real-Time PCR System
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The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.
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ViiA™ 7 Real-Time PCR System
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The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.
Learn More
Step 4: Allelic Discrimination
Perform an endpoint plate read using Applied Biosystems® Real-Time PCR Systems; TaqMan® SNP Genotyping Assay MGB probes provide a fluorescence signal for the amplification of each allele.
Products for Step 4: Allelic Discrimination
Instruments & Accessories
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7900HT Fast Real-Time PCR System
-
The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.
-
StepOnePlus™ Real-Time PCR System
-
Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
-
StepOne™ Real-Time PCR System
-
Applied Biosystems’ 48-well low-throughput real-time PCR instrument, the StepOne™ Real-Time PCR System, makes it simple and easy to get publication ready high quality data on day one. This remarkably simple real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers new to real-time PCR.
-
7500 Fast Real-Time PCR System
-
The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
-
7300 Real-Time PCR System
-
Get the Applied Biosystems advantage at an affordable price; the 7300 Real-Time PCR System balances exceptional quality with budget sensitivity. This 3rd-generation system is built on over 10 years of real-time expertise and is accurate, reliable, and easy to use. Advanced multicolor detection capabilities allow you to perform a wide variety of applications with the highest confidence in results.
-
ViiA™ 7 Real-Time PCR System
-
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.
Step 5: Analyze Data
The plotted fluorescence signals indicate which alleles are in each sample. Make manual allele calls or review automatic allele calls, and then convert allele calls to genotypes.
Products for Step 5: Analyze Data
Software
- TaqMan® Genotyper Software
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TaqMan® Genotyper Software is for use with TaqMan® SNP Genotyping Assays (Pre-Designed, Custom & DME) in combination with 48-, 96- & 384-well microtiter plates, and OpenArray® Genotyping Plates and offers a state-of-the-art genotype calling algorithm, an intuitive user interface, and enhanced multi-plate analysis features.
Product Bulletins
- TaqMan® Genotyping Assays
- Custom TaqMan® Genotyping Assays
- TaqMan® Drug Metabolism Genotyping Assays
- SNPbrowser™ Software v3.5
- Mouse SNPbrowser™ Software v1.0
Posters
Application Notes
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