Gene Expression Analysis Using TaqMan® Assays in 384-Well Microfluidic Cards

Step 1: Isolate RNA

Products for Step 1: Isolate RNA

TaqMan® Gene Expression Cells-to-CT™ Kit

This kit enables researchers to easily perform expression analysis using cultured cells without purification of RNA. The kit includes reagents for cell lysis and RNA preservation, genomic DNA removal, and reverse transcription (RT). The kit also includes TaqMan® Gene Expression Master Mix for use in subsequent real-time PCR analysis.

TaqMan® PreAmp Cells-to-CT™ Kit

Designed for use with the TaqMan Gene Expression Cells-to-CT Kit, this kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical  XenoRNA™ control and corresponding TaqMan Gene Expression Assay to monitor the effects of PCR inhibition.

TaqMan® Cells-to-CT™ Control Kit

Designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit, this kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition.

TaqMan® Fast Cells-to-CT™ Kit

Use Fast-cycling real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification. Quickly and easily lyse cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. The kit includes reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for subsequent real-time PCR analysis.

Step 2: Reverse Transcribe

Products for Step 2: Reverse Transcribe

High Capacity RNA-to-cDNA™ Kit

High Capacity RNA-to-cDNA™ Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.

Step 3: Select Amplification Reagents

Products for Step 3: Select Amplification Reagents

TaqMan® Fast Advanced Master Mix

TaqMan® Fast Advanced Master Mix is designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes).

TaqMan® PreAmp Master Mix

TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and the TaqMan® Gene Expression Master Mix. The two work together to provide the best results in preamplification and quantitation of mRNA. 

TaqMan® Universal Master Mix II

TaqMan® Universal Master Mix II brings sensitivity and precision across a broad range of input target quantities, reliable detection of low copy number targets, and accurate quantification to discriminate subtle differences in target abundance. 

TaqMan® PreAmp Pools

TaqMan® PreAmp Pools are pre-pooled sets of TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Array Gene Signature Cards. TaqMan® PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are available corresponding to fixed TaqMan® ArrayHuman and Rat GPCR, and Human and Mouse Stem Cell Pluripotency Gene Signature Cards.

Step 4: Select Assays

Products for Step 4: Select Assays

Custom TaqMan® Array Micro Fluidic Card

The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to fill the card. This low- to medium- throughput micro fluidic card allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the card. The TaqMan® Custom Array is completely customizable. A minimum order of 10 cards is required for all formats.

TaqMan® Gene Signature Arrays

These 384-well micro fluidic cards are ideal for microarray validation or other large-scale studies. Each contains a set of pre-selected TaqMan® Assays relevant for specific pathways, biomarker sets, or disease states, including:

• Apoptosis
• Angiogenesis
• Alzheimer's Disease
• Endogenous Controls
• Immune System and Inflammation
• Signal Transduction
• Stem Cells

Step 5: Perform Real-Time PCR Amplifications

Products for Step 5: Perform Real-Time PCR Amplifications

7900HT Fast Real-Time PCR System

TThe 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading — and also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

ViiA™ 7 Real-Time PCR System

The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.

Step 6: Run & Analyze

Products for Step 6: Run & Analyze

Software

DataAssist™ Software

DataAssist™ Software is an easy to use data analysis tool that utilizes the Comparative Ct (ddCt) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.

RealTime StatMiner® Software

RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.