Gene Expression Analysis Using TaqMan® Assays in 384-Well Plates
TaqMan®Array 384-well Plates are the right tool for validating tens or hundreds of microarray hits because they can be customized to include up to 384 of those hits in one easy-to-use 384-well plate. Using individual assays, or even SYBR®-based assays, to look at 12 to 384 targets can quickly become unmanageable and expensive. TaqMan® Array Plates enable you to accomplish the validation necessary to arrive at the right answer easily and affordably.
Products
Experimental Workflow: Find the Products You Need for Every Step
This kit enables researchers to easily perform expression analysis using cultured cells without purification of RNA. The kit includes reagents for cell lysis and RNA preservation, genomic DNA removal, and reverse transcription (RT). The kit also includes TaqMan® Gene Expression Master Mix for use in subsequent real-time PCR analysis.
The TaqMan® PreAmp Cells-to-CT™ Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification. For situations in which limited sample availability restricts the number of real-time PCR reactions, this kit enables experiments that would otherwise not be possible.
Designed for use with the TaqMan Gene Expression Cells-to-CT Kit, this kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artifical XenoRNA™ control and corresponding TaqMan Gene Expression Assay to monitor the effects of PCR inhibition.
Use Fast-cycling real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification. Quickly and easily lyse cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. The kit includes reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for subsequent real-time PCR analysis.
Step 2: Reverse Transcribe
The High Capacity RNA-to-cDNA Kit provides all the necessary components to reverse transcribe RNA into cDNA. (Note: this step can be omitted if a one-step RT-PCR protocol is used.)
High Capacity RNA-to-cDNA Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.
Step 3: Select Amplification Reagents
Select from a broad range of amplification reagents optimize for reliable detection of low copy number targets, and accurate quantification to discriminate subtle differences in target abundance.
Products for Step 3: Select Amplification Reagents
TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and the TaqMan® Gene Expression Master Mix. The two work hand in hand to provide the best results in preamplification and quantitation of mRNA.
TaqMan® Universal Master Mix II brings sensitivity and precision across a broad range of input target quantities, reliable detection of low copy number targets, and accurate quantification to discriminate subtle differences in target abundance
TaqMan® PreAmp Pools are pre-pooled sets of TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan® PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are available corresponding to fixed TaqMan® Human and Rat GPCR, and Human and Mouse Stem Cell Pluripotency Gene Signature Arrays.
Step 4: Select Assays
Select the TaqMan® Assay configuration to fit your research goals.
Similar to Custom TaqMan® Array Plates, but can be configured with any TaqMan® Gene Expression Assays, including custom assays designed to your target sequences and made-to-order assays on a 96-well plate format.
Nine different plate formats to choose from
20 plate minimum
Step 5: Perform Real-Time PCR Amplification
The real-time PCR can be run on any Applied Biosystems 7900HT Real-Time PCR System with a 384-well block.
Products for Step 5: Perform Real-Time PCR Amplification
The only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading, and also offers optional fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.
The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.
DataAssist™ Software is an easy to use data analysis tool that utilizes the Comparative Ct (ddCt) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.
