Step 1: Isolate RNA

Products for Step 1: Isolate RNA

TaqMan® Gene Expression Cells-to-CT™ Kit

This kit enables researchers to easily perform expression analysis using cultured cells without purification of RNA. The kit includes reagents for cell lysis and RNA preservation, genomic DNA removal, and reverse transcription (RT). The kit also includes TaqMan® Gene Expression Master Mix for use in subsequent real-time PCR analysis.

TaqMan® PreAmp Cells-to-CT™ Kit

The TaqMan® PreAmp Cells-to-CT™ Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA  purification. For situations in which limited sample availability restricts the number of real-time PCR reactions, this kit enables experiments that would otherwise not be possible.

TaqMan® Cells-to-CT™ Control Kit

Designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit, this kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition.

TaqMan® Fast Cells-to-CT™ Kit

Use Fast-cycling real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification. Quickly and easily lyse cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. The kit includes reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for subsequent real-time PCR analysis.

Step 2: Reverse Transcribe

Products for Step 2: Reverse Transcribe

High Capacity RNA-to-cDNA™ Kit

High Capacity RNA-to-cDNA™ Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.

Step 3: Select Amplification Reagents

Products for Step 3: Select Amplification Reagents

TaqMan® Fast Advanced Master Mix

TaqMan® Fast Advanced Master Mix is designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes).

TaqMan® PreAmp Master Mix

TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and the TaqMan® Gene Expression Master Mix. The two work together to provide the best results in preamplification and quantitation of mRNA. 

TaqMan® Universal Master Mix II

TaqMan® Universal Master Mix II brings sensitivity and precision across a broad range of input target quantities, reliable detection of low copy number targets, and accurate quantification to discriminate subtle differences in target abundance. 

TaqMan® PreAmp Pools

TaqMan® PreAmp Pools are pre-pooled sets of TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan® PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are available corresponding to TaqMan® Human and Rat GPCR, and Human and Mouse Stem Cell Pluripotency Gene Signature Plates.

Step 4: Select Plate Format

Products for Step 4: Select Plate Format

TaqMan® Array Gene Signature Plates

These 96-well plates come in Fast and standard formats, and are pre-configured with the most appropriate TaqMan® Gene Expression Assays for a specific biological process, pathway, or disease state. Each plate contains predefined assays and endogenous controls dried-down in the wells, ready for accurate assessment of an entire gene signature in one simple experiment.

Taqman® Array Gene Signature  Plates contain sets of pre-selected TaqMan® Assays relevant for specific pathways, biomarker sets, or disease states including:

• Apoptosis
• Biomarkers Related Pathway
• Cancer
• Cell Cycle Proliferation and Regulation
• Development and Stem Cells
• ECM Matrix and Adhesion
• Endogenous Controls
• Immune System and Inflammation
• Neurology
• Reproduction
• Signal Transduction
• Toxicology and Drug Metabolism
• and more

Custom TaqMan® Array Plates

TaqMan® Plates are a cost-effective and convenient 96-well format. Customize any of the nine available formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount of specified gene targets expressed in human, mouse, rat, canine, and rhesus species.

• Select any inventoried TaqMan® Gene Expression Assays to be loaded into your custom plate
• Available in fast and standard cycling formats

TaqMan® Custom Plating Service

TaqMan® Custom Plating service offers the convenience of pre-plated TaqMan® Gene Expression Assays, Custom Assays, and Custom Probe/Primer Sets in 96- or 384-well plates.

• Similar to Custom TaqMan® Array Plates, but can be configured with any TaqMan® Gene Expression Assays, including custom assays designed to your target sequences and made-to-order assays on a 96-well plate format
  
• Nine different plate formats to choose from
• 20 plate minimum

Step 5: Perform Real-Time PCR Amplification

Products for Step 5: Perform Real-Time PCR Amplification

StepOnePlus™ Real-TimePCR System

This system makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4-color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

7300 Real-Time PCR System

Get the Applied Biosystems advantage at an affordable price. The 7300 Real-Time PCR system balances exceptional quality with budget sensitivity.

7500 Real-Time PCR System

The Applied Biosystems® 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third-generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

7500 Fast Real-Time PCR System

The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.

7900HT Fast Real-Time PCR System

The only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading, and also offers optional fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

Step 6: Analyze Data

Products for Step 6: Analyze Data

Software

DataAssist™ Software

DataAssist™ Software is an easy to use data analysis tool that utilizes the Comparative Ct (ddCt) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.

RealTime StatMiner® Software

RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.