Gene Expression Analysis Using TaqMan® Assays in Single Tubes

Step 1: Isolate RNA

Products for Step 1: Isolate RNA

TaqMan® Gene Expression Cells-to-CT™ Kit

This kit enables researchers to easily perform expression analysis using cultured cells without purification of RNA. The kit includes reagents for cell lysis and RNA preservation, genomic DNA removal, and reverse transcription (RT). The kit also includes TaqMan® Gene Expression Master Mix for use in subsequent real-time PCR analysis.

TaqMan® PreAmp Cells-to-CT™ Kit

The TaqMan® PreAmp Cells-to-CT™ Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification. For situations in which limited sample availability restricts the number of real-time PCR reactions, this kit enables experiments that would otherwise not be possible.

TaqMan® Cells-to-CT™ Control Kit

Designed for use with the TaqMan® Gene Expression Cells-to-CT™ Kit, this kit contains an endogenous control (ACTB) to normalize for sample loading variability and an artificial XenoRNA™ control and corresponding TaqMan® Gene Expression Assay to monitor the effects of PCR inhibition. 

TaqMan® Fast Cells-to-CT™ Kit

Use Fast-cycling real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification. Quickly and easily lyse cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity. The kit includes reverse transcription (RT) reagents and TaqMan® Fast Universal PCR Master Mix for subsequent real-time PCR analysis.

Step 2: Reverse Transcribe

Products for Step 2: Reverse Transcribe

High Capacity RNA-to-cDNA™ Kit

High Capacity RNA-to-cDNA™ Kit provides high quality reverse transcription that delivers a streamlined workflow with maximum sensitivity and dynamic range.

Step 3: Select Amplification Reagents

Products for Step 3: Select Amplification Reagents

TaqMan® Fast Advanced Master Mix

TaqMan® Fast Advanced Master Mix is designed to match or exceed the performance of standard master mixes, delivering shorter run times (<40 minutes).

TaqMan® PreAmp Master Mix

TaqMan® PreAmp Master Mix preamplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions. TaqMan® PreAmp Master Mix Kit consists of TaqMan® PreAmp Master Mix and the TaqMan® Gene Expression Master Mix. The two work together to provide the best results in preamplification and quantitation of mRNA. 

TaqMan® Universal Master Mix II

TaqMan® Universal Master Mix II brings sensitivity and precision across a broad range of input target quantities, reliable detection of low copy number targets, and accurate quantification to discriminate subtle differences in target abundance. 

Step 4: Select Assays

Products for Step 4: Select Assays

TaqMan® Gene Expression Assays

Applied Biosystems offers more than 1,000,000 TaqMan® Gene Expression Assays, the most comprehensive set of pre-designed real-time PCR assays available. All TaqMan® Gene Expression Assays have been designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization.

Custom TaqMan® Gene Expression Assays

Order all-in-one-tube Custom TaqMan® Gene Expression Assays to perform gene expression studies on any gene or splice variant in any organism. Three different assay sizes are available. For your convenience, either submit your target sequence of interest or primer and probe sequences that you have already designed. Assays can also be generated for sequence detection of specific genomic DNA.

TaqMan® Endogenous Controls

Eliminate months of assay design, formulation, and testing by using TaqMan® Endogenous Controls as your controls to quantify gene expression. This convenient collection of pre-designed probe and primer sets enables you to normalize the amount of sample RNA or DNA in a reaction. Now you can avoid all the trial-and-error of selecting controls for most common human, mouse, rat, and eukaryotic genes.

Step 5: Perform Real-Time PCR Amplification

Products for Step 5: Perform Real-Time PCR Amplification

StepOnePlus™ Real-TimePCR System

This system makes it simple and easy to get publication-ready high-quality real-time PCR results on day one.  This remarkably simple 96-well, 4-color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels. 

7300 Real-Time PCR System

Get the Applied Biosystems advantage at an affordable price.  The 7300 Real-Time PCR system balances exceptional quality with budget sensitivity.

7500 Real-Time PCR System

A powerful platform for labs requiring superior performance and maximum dye versatility. This third-generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

7500 Fast Real-Time PCR System

The 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.

7900HT Fast Real-Time PCR System

The only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading, and also offers optional fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.

ViiA™ 7 Real-Time PCR System

The ViiA™ 7 Real-Time PCR System combines all the qPCR features you want in a single high performance instrument to optimize your productivity. With a simplified workflow, intuitive software, touch-screen interface, and one-button protocols for error minimization, this system offers exceptional reproducibility with minimal well-to-well and instrument-to-instrument variation. Full compatibility with any standard or Fast-cycling 384- or 96-well plates and TaqMan® Array Microfluidic Cards and Assays makes high-productivity real-time PCR accessible to any size lab.

Step 6: Analyze Data

Products for Step 6: Analyze Data

Software

DataAssist™ Software

DataAssist™ Software is an easy-to-use data analysis tool that utilizes the Comparative Ct (ddCt) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.

RealTime StatMiner® Software

RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you though every step of analysis, giving you accurate results in minutes.