TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

The The QuantStudio™ 12K Flex Real-Time PCR System helps streamline targeted discovery and validation, pathogen detection and biomarker identification and screening, at a scale previously unattainable. Achieve the highest sample throughput for mid-density gene expression—in a single day, one user can generate >42,000 gene expression data points on the OpenArray® Real-Time PCR System, without the use of robotics.

More replicates—each plate generates over 2,600 data points, enabling technical replicates for better statistical power
Simple workflows—each QuantStudio™ 12K Flex OpenArray® Plate, encased in an alloy bottom for easy handling, comes preloaded with the assays of your choice—just add sample and master mix, cycle and image
Experimental flexibility—specify your desired inventoried TaqMan® Assays, and select the sample/assay format that best meets your project needs
Reliable and economical—uses gold standard TaqMan® MGB probe chemistry in a nanofluidic design that reduces reagent usage

Products
TaqMan® OpenArray® Gene Expression Workflow on QuantStudio™ 12K Flex System: Find the Products You Need for Every Step
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Step 1: Prepare Sample

Isolate and purify genomic DNA.

Products for Step 1: Prepare Sample

MagMAX™ DNA Multi-Sample Kit

MagMAX™ DNA Multi-Sample Kits are designed for rapid purification of high-quality genomic DNA from a variety of sample types including blood and blood cards, tissue, mouse tails, buccal swabs, buffy coats, and cultured cells. These purification kits use MagMAX™ magnetic bead-based nucleic acid isolation technology to produce high yields of purified DNA, free from inhibitors that may affect downstream reactions such as genotyping assays, CE and next-generation sequencing, and high resolution melting analysis. MagMAX™ kits are available in both tube format (for manual processing) and in 96-well plate format (compatible with automated processing).

Step 2: Select Assays

Select inventoried TaqMan® probes and configure the plate layout using the downloadable workbook tool. TaqMan® OpenArray® Real-Time PCR Plates are delivered with assays dried down in the through-holes you specify. Mix DNA samples with Master Mix in OpenArray® 384-well sample plate.

Products for Step 2: Select Assays

TaqMan® OpenArray® Real-Time PCR Plates

TaqMan® OpenArray® Real-Time PCR Plates deliver world-class TaqMan® Assays in a more flexible, high-throughput format. Five customizable plate formats allow you to select from over 61,000 inventoried TaqMan® Gene Expression Assays, which are pre-loaded onto the OpenArray® Plates. Each TaqMan® OpenArray® Real-Time PCR Plate contains 3,072 through-holes and can be configured using 5 different formats. OpenArray® plates compatible with QuantStudio™ 12K Flex Real-Time PCR system will be coming soon and available only on www.lifetechnologies.com

Step 3: Cycle & Image

Load sample mixes onto TaqMan® OpenArray® Real-Time PCR Plate using the QuantStudio 12K Flex OpenArray® AccuFill™ System. The plate is encased with an alloy bottom for easy handling. Simply apply the adhesive lid and fill with immersion fluid. Perform a real-time plate read using the QuantStudio™ 12K Flex system.

Products for Step 3: Cycle & Image

New! QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

The QuantStudio™ 12K Flex Real-Time PCR Instrument and QuantStudio™ 12K Flex OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.

Step 4: Analyze Data

Acquire, analyze and manage data quickly and easily. Amplification curves are presented for review, and data is easily modified to calculate Ct and Tm as well as ΔCt and ΔΔCt measurements.

Products for Step 4: Analyze Data

QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

The QuantStudio™ 12K Flex Real-Time PCR Instrument and QuantStudio™ 12K Flex OpenArray® AccuFill™ System combination can process hundreds to thousands of samples per day, enabling mid-density, high-throughput results with ease of use and without robotics.
Publications & Literature
Literature
Publications
- High throughput, nanoliter quantitative PCR Publication: Drug Discovery Today, 2006
  Authors: Brenan, et al.
- Nanoliter high throughput quantitative PCR Publication: Nucleic Acids Research, 2006
  Authors: Morrison, et al.
- Advances in molecular phytodiagnostics – new solutions for old problems Publication: European Journal of Plant Pathology (2006) 116:1–19
  Authors: Mumford, et al.
- High-Throughput Functional Genomic Methods to Analyze the Effects of Dietary Lipids Publication: Current Pharmaceutical Biotechnology, 2006
  Authors: Puskás, et al.
- Quantitative multiplex detection of plant pathogens using a novel ligation probe-based system coupled with universal, high-throughput real-time PCR on OpenArrays™. Publication: BMC Genomics 2007, 8:276
  Authors: van Doorn, et al.
- Development and Experimental Validation of a Predictive Threshold Cycle Equation for Quantification of Virulence and Marker Genes by High-Throughput Nanoliter-Volume PCR on the OpenArray Platform Publication: Applied and Enviromental Microbiology, June 2008, p. 3831–3838
  Authors: Stedtfeld, et al.
- Toxicogenomics screening of small molecules using high-density, nanocapillary real-time PCR Publication: International Journal of Molecular Medicine, 2009
  Authors: Vass, et al.
- Nanoliter high-throughput RT-qPCR: a statistical analysis and assessment Publication: BioTechniques 2009
  Authors: Dixon, et al.
- Comparison of Breast Cancer to Healthy Control Tissue Discovers Novel Markers with Potential for Prognosis and Early Detection Publication: PLoS ONE, 2010, 5(2)
  Authors: Schummer M, et al.
- Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts using a novel Internal Amplification Control approach on Biotrove OpenArrays™ Publication: Appl Environ Microbiol., 2009
  Authors: Van Doorn, R., et al.
- Nanoliter high-throughput PCR for DNA and RNA profiling Publication: Methods Mol Bio. 496:161–74
  Authors: c. Brenan, C. J., et al.

TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

Products

TaqMan® OpenArray® Gene Expression Workflow on QuantStudio™ 12K Flex System: Find the Products You Need for Every Step

Click the arrows above to view products

Step 1: Prepare Sample

Products for Step 1: Prepare Sample

Step 2: Select Assays

Products for Step 2: Select Assays

Step 3: Cycle & Image

Products for Step 3: Cycle & Image

Step 4: Analyze Data

Products for Step 4: Analyze Data

Publications & Literature

Literature

Publications

Contact Us

TaqMan® Gene Expression Assays

For More Information

TaqMan® Gene Expression Using the QuantStudio™ 12K Flex Real-Time PCR System with OpenArray® Block

Products

TaqMan® OpenArray® Gene Expression Workflow on QuantStudio™ 12K Flex System: Find the Products You Need for Every Step

Click the arrows above to view products

Step 1: Prepare Sample

Products for Step 1: Prepare Sample

Step 2: Select Assays

Products for Step 2: Select Assays

Step 3: Cycle & Image

Products for Step 3: Cycle & Image

Step 4: Analyze Data

Products for Step 4: Analyze Data

Publications & Literature

Literature

Publications

Contact Us

TaqMan® Gene Expression Assays

For More Information

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