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miRNA Quantitation

Workflow Features

  • Ideal for quantitating individual miRNAs from a broad assortment of species, including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis
  • Each kit includes the TaqMan® MicroRNA Assay and reverse transcription primer specific for the mature miRNA target of interest
  • Assays are continuously added for human, mouse, and rat species to stay aligned with the Sanger miRBase Registry

How It Works
TaqMan® MicroRNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay-based real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.

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