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Chromatin Immunoprecipitation Sequencing (ChIP-Seq)

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Figure 1

DNA enrichment by ChIP and SOLiD™ fragment library construction

Feature SOLiD™ System Microarray
Resolution > 700 million sequence tags/run ~ 6.5 million oligos/array
Genome Coverage UNLIMITED and hypothesis free LIMITED by probe design
Risk of Cross-Hybridization None Between closely related elements
Multiplexing YES NO

Comparison of ChIP Detection Platforms

Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. This technique gives a picture of the protein-DNA interactions that occur inside the nucleus of living cells or tissues. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states.

Microarray-based ChIP studies are restricted to a fixed number of probes
Currently, the most common method for genome-wide analysis of DNA-binding proteins is Chip-on-chip (ChIP-chip), which combines chromatin immunoprecipitation with DNA microarrays. The major limitation of this hybridization-based method is that microarrays are restricted to a fixed number of probes, thereby introducing bias.

ChIP-Seq with the SOLiD™ System

SOLiD™ System-based ChIP-Sequencing (ChIP-Seq) overcomes the limitation of microarrays by combining chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing, offering a highly sensitive, hypothesis-neutral method to accurately characterize the protein-DNA interactions of an entire genome. The SOLiD™ System’s throughput and flexible slide format allow you to analyze both normal and diseased samples in a single run. The SOLiD™ ChIP-Seq Kit is optimized for genome-wide ChIP analysis from as little as 10,000 cells and library construction using 1-10 ng of DNA. Magnetic bead capture technology reduces ChIP workflow to just 5 hours, is compatible with multichannel pipetting and improves reproducibility.

Experimental Workflow: Find the Products You Need for Every Step

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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.