Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
|Resolution||> 700 million sequence tags/run||~ 6.5 million oligos/array|
|Genome Coverage||UNLIMITED and hypothesis free||LIMITED by probe design|
|Risk of Cross-Hybridization||None||Between closely related elements|
Microarray-based ChIP studies are restricted to a fixed number of probes
Currently, the most common method for genome-wide analysis of DNA-binding proteins is Chip-on-chip (ChIP-chip), which combines chromatin immunoprecipitation with DNA microarrays. The major limitation of this hybridization-based method is that microarrays are restricted to a fixed number of probes, thereby introducing bias.
ChIP-Seq with the SOLiD™ SystemSOLiD™ System-based ChIP-Sequencing (ChIP-Seq) overcomes the limitation of microarrays by combining chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing, offering a highly sensitive, hypothesis-neutral method to accurately characterize the protein-DNA interactions of an entire genome. The SOLiD™ System’s throughput and flexible slide format allow you to analyze both normal and diseased samples in a single run. The SOLiD™ ChIP-Seq Kit is optimized for genome-wide ChIP analysis from as little as 10,000 cells and library construction using 1-10 ng of DNA. Magnetic bead capture technology reduces ChIP workflow to just 5 hours, is compatible with multichannel pipetting and improves reproducibility.
Experimental Workflow: Find the Products You Need for Every Step
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