De Novo Sequencing
The initial generation of the primary genetic sequence of a particular organism is called de novo sequencing. A detailed genetic analysis of any organism is possible only after de novo sequencing has been performed.
With its long read lengths and high accuracy, capillary electrophoresis-based sequencing is the gold standard technology for de novo projects. However, the throughput of these systems makes de novo assembly of most organisms a lengthy and costly endeavor. Next-generation sequencing technologies hold great promise in reducing the time and cost of sequencing.
De novo assembly of small organisms using the SOLiD™ System
The longer read lengths and new data analysis software of the SOLiD™ System enable the de novo assembly of small organisms. Researchers have demonstrated the assembly of 50bp reads into a scaffold that can then be filled in with capillary electrophoresis-based sequencing.
SOLiD™ System facilitates the assembly of difficult genomic regions.
For larger organisms, capillary electrophoresis-based sequencing is the preferred platform for scaffold generation. Once a scaffold is created the SOLiD™ System can be used to fill in the gaps at high coverage.
