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SOLiD™ System Accuracy

The SOLiD™ System enables unsurpassed accuracy and sensitivity to detect biological variation across multiple samples in a single run.

  • Superior accuracy – greater than 99.94%
    High-quality bases, resulting in fewer false positives and subsequently less validation.

Experience SOLID Accuracy


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Sequencing Reads

See for Yourself

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SOLiD Base Quality

SOLiD™ System Base Quality

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SNP Detection

SOLiD™ System Power of
SNP Detection
For more information visit: http://www.broadinstitute.org/igv


 View white paper regarding:
2 Base Encoding and its application to annotations, error detection, and error correction. (PDF)		  

Low Raw Error Rate

The new SOLiD™ Accuracy Enhancement Tool (SAET) enhances the SOLiD™ System's unsurpassed accuracy and throughput by increasing mappability of reads and length of contigs in de novo assembly of prokaryotic genomes, giving you a more finished sequence assembly.

Benefits Applications
  • Accurate results
    SAET has demonstrated significant improvements in mapping and SNP calling and delivers increases in contig lengths in de novo assembly by up to 3 times
  • Unsurpassed performance and scalability
    SAET can scale with linear performance on various datasets, including a large spectrum of genome sizes and complexities as well as coverages and read lengths*
  • Data reliability
    SAET reduces the error rate to 1% without overcorrecting true SNPs
  • De Novo Sequencing
    Increases length of contigs by up to 3 times for de novo assembly (for bacterial genomes N50=20 kb).
  • Targeted Resequencing
    Helps reduce false positive and false negative SNP calls; due to color space, SAET can distinguish between true SNPs and sequencing error, ultimately reducing the overcorrection of real sequence variation.
  • Whole Transcriptome Analysis
    Enables increased read mappability, sensitivity, and alignment.
* SAET is limited to datasets where the length of sequenced (enriched) segment of DNA is smaller than 200 Mb; however, the limit on genome size can be increased for those with larger RAM.

Find more information and download the SOLiD™ Accuracy Enhancement Tool from the SOLiD™ Software Community site.

Additional accuracy software tools:

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