Cell Line Identification

The misidentification and cross contamination of laboratory cell lines is a common problem effecting many facets of biomedical research, including stem cell and cancer biology It is estimated that over 20% of all established lines are misclassified by cell type or species of origin. The implications are far reaching, including invalidation of published data and lost time, money and effort. As a result, it is recommended that all cell lines, regardless of origin, should be tested upon receipt and at regular intervals between passages.

Molecular profiling by short tandem repeats (STR or microsatellite markers) is a fast, affordable and highly discriminating method to authenticate a cell line. Due to the highly polymorphic nature of each STR loci, every cell line will have a unique digital bar code that can distinguish it from other sources. A common set of STR loci are widely used for forensic applications and can be used for intra-species authentication.

The AmpFℓSTR® Identifiler® PCR Amplification Kit is an ideal solution for rapid screening of cultured stem cells.

• Highly Informative: A multiplex STR kit which contains 15 loci and 1 sex determination marker (Amelogenin) for high resolution screening to detect intra-species cross contamination.
• Highly Sensitive: Capable of detecting less than 100 picograms of genomic DNA (~ 15 diploid cells).
• High Throughput: Based on 5 dye chemistry which allows higher density multiplexing with better spectral resolution to minimize false positive calls due to fluorescent artifacts.
• Integrated Workflow: Compatible with the Applied Biosystems 3130 Genetic Analyzer, GeneScan™ 500 LIZ® Size Standard and GeneMapper® ID Software.