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Epigenetic Monitoring

Epigenetic factors are known to play a critical role in regulating stem cell differentiation by controlling when and how transcription factor bind to promoters or other regulatory elements. Understanding this regulatory mechanism will provide insight into how cells, containing the same genetic material, exhibit significantly different characteristics.

Genetic modifications include the following:
  • Genomic imprinting
  • X-chromosome inactivation
  • DNA methylation
  • Histone modifications
These modifications possess unique molecular signatures that are characteristic by cell state (self-renewal vs. differentiated) or cell type (embryonic, early progenitors or lineage specific cells).

DNA Methylation Analysis is used to identify patterns of methylation by relying on bisulfite treatment of methylated genomic DNA to convert unmethylated cytosine to uracil. Methylated cystosine residues remain unchanged. Treated or untreated samples can be directly compared to identify methylated sites and unique patterns of methylation within a site.

The Applied Biosystems methylSEQr™ Bisulfite Conversion Kit Workflow is based on PCR amplification and direct sequencing to offer greater resolution to identify unique molecular signatures. The methylSEQr™ kit protocol consists of the following:

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For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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