Gene Expression Profiling

Step 1: Isolate Cells

Products for Step 1: Isolate Cells

Invitrogen Products

View more reagents, kits and bench-top devices on invitrogen.com

Dynabeads® SSEA-4
hESC can be differentiated into neural-, mesenchymal- and hematopoetic stem cells. This product is intended for the magnetic depletion of SSEA-4+ undifferentiated ESC from a differentiated cell population to increase purity and enable safer cell therapies. The purified differentiated cells are bead- and antibody free and are suitable for any downstream application.

Dynal® CD34 Progenitor Cell Selection System
Use this kit to isolate a high purity and yield of human CD34+ progenitor stem cells. Positively isolated cells are bead and antibody-free, phenotypically unaltered and suitable for any downstream applications including flow cytometry, functional studies and cell culture to produce dendritic cells (DC’s).

Step 2: Lyse and Reverse-Transcribe

Products for Step 2: Lyse and Reverse-Transcribe

TaqMan® Gene Expression Cells-to-CT™ Kit

This kit enables researchers to easily perform expression analysis using cultured cells without purification of RNA. The kit includes reagents for cell lysis and RNA preservation, genomic DNA removal, and reverse transcription (RT). The kit also includes TaqMan® Gene Expression Master Mix for use in subsequent real-time PCR analysis.

TaqMan® PreAmp Cells-to-CT™ Kit

Perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification. The protocol includes an intermediate amplification step (pre-amplification) between reverse transcription and real-time PCR in which cDNA is enriched for up to 100 gene targets of the user’s choice without amplification bias.

Step 3: Pre-Amplify

Products for Step 3: Pre-Amplify

TaqMan® PreAmp Cells-to-CT™ Kit

Perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification. The protocol includes an intermediate amplification step (pre-amplification) between reverse transcription and real-time PCR in which cDNA is enriched for up to 100 gene targets of the user’s choice without amplification bias.

Step 4: Amplify

Products for Step 4: Amplify

TaqMan® Stem Cell Pluripotency Array

The Human Stem Cell Pluripotency Array contains a well-defined set of validated gene expression markers to characterize embryonic stem (ES) cell identity and assess phenotypic variations between embryonic stem cell isolates.

TaqMan® Gene Expression Assays

Applied Biosystems offers more than 1,000,000 TaqMan® Gene Expression Assays, the most comprehensive set of pre-designed Real-Time PCR assays available. All TaqMan Gene Expression Assays have been designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization.

TaqMan® Gene Expression Master Mix

The TaqMan® Gene Expression Master Mix is tailored for precise quantification by real-time PCR. It is designed for routine and challenging experiments.

Step 5: Quantify

Products for Step 5: Quantify

StepOnePlus™ Real-Time PCR System

The Applied Biosystems® StepOnePlus™ Real-Time PCR System makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96-well, 4-color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.

7500 Real-Time PCR System

The Applied Biosystems® 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third-generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.

7500 Fast Real-Time PCR System

The Applied Biosystems® 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast System delivers high-quality results in as little as 30 minutes.

7900HT Fast Real-Time PCR System

The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading — and also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT System combined with TaqMan® Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments.