Step 1: Isolate miRNA

Products for Step 1: Isolate miRNA

TaqMan® MicroRNA Cells-to-CT™ Kit

Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan MicroRNA Cells-to-CT Kit makes it possible to perform microRNA (miRNA) expression profiling directly from cultured cells without RNA purification.

Ambion® mirVana™ miRNA Isolation Kit

Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

Step 2: Convert to cDNA

Products for Step 2: Convert to cDNA

TaqMan® MicroRNA Reverse Transcription Kit

The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

Step 3: Quantitate miRNA Expression

Products for Step 3: Quantitate miRNA Expression

TaqMan® MicroRNA Assays

TaqMan® MicroRNA Assays quantitate microRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan probes. The assays target only mature microRNAs, not their precursors, ensuring biologically relevant results.

TaqMan® Universal Master Mix II

This kit contains components needed for all 5' nuclease assays using TaqMan®-probe PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

TaqMan® Array MicroRNA Cards

TaqMan® Array MicroRNA Cards are the ideal solution for profiling large numbers of miRNAs. This product requires the use of Megaplex™ Primer Pools for miRNA reverse transcription.  Optional Megaplex™PreAmp Primers can be used for preamplification of limited samples prior to real-time PCR using the TaqMan® Array MicroRNA Card.

Step 4: Perform Functional Analysis

Products for Step 4: Perform Functional Analysis

Ambion® Anti-miR™ miRNA Inhibitors

Ambion® Anti-miR™ miRNA Inhibitors are chemically modified, single stranded nucleic acids designed to specifically bind to and inhibit endogenous microRNA (miRNA) molecules. These ready-to-use inhibitors can be introduced into cells using transfection or electroporation parameters similar to those used for siRNAs, and enable detailed study of miRNA biological effects.

Ambion® Pre-miR™ miRNA Precursors

Ambion® Pre-miR™ miRNA Precursor Molecules are small, chemically modified double-stranded RNA molecules designed to mimic endogenous mature miRNA molecules. These ready-to-use miRNA mimics, which are similar to but not identical to siRNAs, can be introduced into cells using transfection or electroporation parameters identical to those used for siRNAs and enable detailed study of miRNA biological effects via gain-of-function experiments.

Step 5: Analyze Data

Products for Step 5: Analyze Data

DataAssist™ Software

DataAssist™ Software is an easy to use data analysis tool that utilizes the Comparative CT (ddCT) method to rapidly and accurately quantitate relative gene expression across a large number of genes and samples.