Protein Expression
Protein biomarkers are routinely used to characterize stem cells by cell state, cell type and function. Methods such as immunostaining, Western Blot analysis and FACS have been used to identify, enrich, localize and quantify singular proteins in pluripotent and differentiated cells. However, future needs in stem cell biology require greater throughput and sensitivity to enable global and target-specific analysis. An example of a new target-specific analysis approach is our new TaqMan® Protein Assays.
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Publications & Literature
References
- Poster: Cardoso, H.A. and Hart, R.P., Establishing standard assays for quality control and identity traceability of human embryonic stem cells (2007)
- An Open Letter Regarding the Misidentification and Cross-Contamination of Cell Lines
- White Paper: Eradication of Cross-Contaminated Cell Lines
Guides
- Molecular Analysis Guide for Stem Cell Researchers
- NIH Approved Cell Line Providers
- TaqMan® Pluripotency Array Assay ID Information
- Commonly Used Stem Cell Markers
Product Information
- Product Bulletin: TaqMan® Protein Expression Assays
- Protein Ligation Technology
- Poster: Novel Protein Expression Assays using qPCR for the Detection and Relative Quantification of Protein Markers in Stem Cells
- Poster: An integrated quantitative PCR approach for monitoring gene and protein expression in human pluripotent and differentiated cells
- Poster: A Novel Quantitative PCR-Based Protein Detection Method for OCT3/4, NANOG, SOX2, and LIN28 in Human Germ Cell Tumors (GCTs)
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