Waterborne Pathogen Detection
Detecting and monitoring waterborne pathogens in recreational and drinking water supplies is essential to protect against preventable human infection. Rapid methods such as qPCR can deliver results, from sample prep to data analysis, in less than a few hours. Delivering accurate, same-day results can greatly improve the ability to respond to contamination events in time to contain the health risk to the public.
Compared to traditional methods such as culture, qPCR-based solutions:
- Reduce time-to-results from 24 hours to less than a few hours
- Increase accuracy and reduce false negatives by using pathogen-specific TaqMan® Assays
- Eliminate lengthy and subjective data analysis
Applied Biosystems® products lead the industry for fixed and custom waterborne pathogen qPCR tests. Even if you are new to qPCR, when you use Applied Biosystems® products, you get expert service and support teams that can help get your testing validated and implemented in minimal time.
Contact us to receive a consultation on how we can help you move from traditional methods over to qPCR quickly and effectively.
Request a Consultation Now
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Products
Experimental Workflow: Find the Products You Need for Every Step
Click the arrows above to view products
Products for Step 1: Nucleic Acid Extraction
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PrepSEQ™ Rapid Spin Sample Preparation Kit
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Use the PrepSEQ™ Rapid Spin Sample Preparation Kit to produce high-quality bacterial DNA samples for PCR-based detection from a wide range of environmental samples.
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MagMAX™ Total Nucleic Acid Isolation Kit
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The MagMAX™ Total Nucleic Acid Isolation Kit is for rapid high throughput purification of DNA and RNA for use in PCR and RT-PCR applications. Suitable for difficult-to-lyse samples, such as Mycobacterium.
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MagMAX™ Express Magnetic Particle Processor
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MagMAX™ Express magnetic particle processors are ideal for researchers who want to automate their MagMAX sample preparation kits. The MagMAX Express platform is the premier automated platform to meet your throughput needs. It seamlessly incorporates the rapid, reliable, and cost efficient magnetic bead-based extraction of nucleic acids that you expect from MagMAX technology.
Products for Step 2: TaqMan® Assays
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TaqMan® E. coli O157:H7 Detection Kit
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The TaqMan® E. coli O157:H7 Detection Kit provides a simple, reliable, and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to E. coli O157:H7.
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TaqMan® Salmonella enterica Detection Kit
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The TaqMan® Salmonella enterica Detection Kit provides a simple, reliable, and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to Salmonella enterica.
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Custom TaqMan® Expression Assays
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Custom TaqMan® Expression Assays are the custom version of our original, gold standard 5' nuclease TaqMan® Assays for relative quantification of RNA expression. Simply provide us with your target sequence of interest, or submit primer/probe sequences of your own design, and soon you'll be ready to perform RNA expression studies on any coding or non-coding RNA in any organism.
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TaqMan® Environmental Master Mix 2.0
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The TaqMan® Environmental Master Mix 2.0 offers accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors. Use this Master Mix to analyze environmental, food and other challenging samples.
Products for Step 3: Detection and Data Analysis
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StepOnePlus™ Real-Time PCR System
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Applied Biosystems’ latest introduction to real-time PCR, the StepOnePlus™ Real-Time PCR System, makes it simple and easy to get publication-ready high-quality real-time PCR results on day one. This remarkably simple 96 well, 4 color, real-time PCR system is designed with a user-friendly, yet powerful, interface for researchers of all experience levels.
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7500 Fast Real-Time PCR System
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The Applied Biosystems® 7500 Real-Time PCR System is a powerful platform for labs requiring superior performance and maximum dye versatility. This third-generation platform features an innovative optical system that enhances sensitivity and lets you access a broader range of fluorophores. The variable excitation capability allows greater sensitivity for longer wavelength (red) dyes.
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RapidFinder™ Express Software
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RapidFinder™ Express Software has been designed for use with the MicroSEQ Pathogen Detection Kits and the Applied Biosystems 7500 Fast Real-Time PCR system. The software is easy to use, guiding you through the food pathogen detection assay procedure and displaying the results in an intuitive format.
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Literature
For research use only. Not intended for any animal or human therapeutic or diagnostic use.