Tutorials & Troubleshooting

Top Tutorials, Guides & Troubleshooting Documents

 DNA Sequencing and Fragment Analysis

•  Getting Started Guides
  • Automated DNA Sequencing Chemistry Guide (3.06MB)
  • Genescan® Reference Guide: Chemistry Reference for the ABI Prism® 310 Genetic Analyzer (1.9MB)
  
  
•  Training
  • DNA Sequencing Training
  • Fragment Analysis Training
  • Training Calender
  
  
•  Fragment Analysis/Genotyping Applications
  • Application Note: AFLP Analysis on Applied Biosystems Capillary Electrophoresis Systems (229KB)
  • Application Note: BAC Fingerprinting on the Applied Biosystems 3730/3730xl DNA Analyzer (3.22MB)
  • Fact Sheet: Microsatellite Analysis on the 3130 Series Systems (491KB)
  • Application Note: Quanititative Multiplex PCR-based Assay: Detection of Large Deletions or Duplications in Genomic DNA Using Quantitative Multiplex PCR-Based Assay on Applied Biosystems Capillary Electrophoresis Systems (5.07MB)
  • Application Note: Relative Fluorescent Quantitation on the 3130 Series Systems (907KB)
  • SNaPShot™ Multiplex Kit
  • SNPlex
  • Application Note: Single-Stranded Conformation Polymorphism (SSCP) on Applied Biosystems Capillary Electrophoresis Systems (319KB)
  • Application Note: Terminal Fragment Length Polymorphism (T-RFLP) on Applied Biosystems Capillary Electrophoresis Systems (216KB)
  
  
•  DNA Sequencing Applications
  • Application Note: A Workflow for Obtaining High Quality Sequencing Data from Bacterial Artificial Chromosome (BAC) DNA (631KB)
  • Application Note: MLST: A Fast, Accurate, and Automated Workflow for Multi Locus Sequence Typing of Bacterial Isolates (551KB)
  • MitoSEQr™ Resequencing System Protocol Summary (788KB)
  • VariantSEQr™ Resequencing System: Protocol Summary (NCBI Search for Primers) (1.54MB)
  
  
•  Instrument Guides
  •  310 Genetic Analyzer
    • Autosampler Calibration (635k)
    • Resolving Communication Problems (1MB)
    • Test CCD 4 Color and Seq Fill Modules (840k)
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  •  3100-Avant and 3100 Genetic Analyzer
    • 3130/3130xl Genetic Analyzers Maintenance, Troubleshoooting, and Reference Guide, Rev. D (3.37MB)
    • 3130/3130xl Genetic Analyzers Getting Started Guide, Rev. C (10.54MB)
    • User Bulletin: Introducing New 96-Well Fast Plate Adapters for Applied Biosystems Capillary Electrophoresis Systems, Rev. C (1.14MB)
    
    
  • 3130 and 3130xl Genetic Analyzer
  • 3730 and 3730xl DNA Analyzer
  
  
•  Analysis Software Guides
  •  GeneMapper® Software v4.0
    • GeneMapper® Software v.4.0 with Multi-User Client-Server Capability (437KB)
    
    
  •  Sequencing Analysis Software
    • Sequencing Analysis Software v.5.3 User Bulletin, Rev. C (382KB)
    • Frequently Asked Questions: KB™ Basecaller v.1.4 (166KB)
    
    
  •  SeqScape® Software
    • Applied Biosystems SeqScape® Software v2.5: Quick Reference Guide: Rev A (333k)
      
    • SeqScape® Software Version 2.5: Frequently Asked Questions: Rev A (334k)
    
    
  •  Troubleshooting
    • Troubleshooting Computer Systems: Windows® 2000 and Windows® XP Computer Systems: Technical Support Bulletin 1 (199KB)
    • Technical Support Bulletin 2: Submitting and Receiving Data to and from Applied Biosystems Genetic Analysis Technical Support, Rev. A (568KB)
  
  

 PCR

•  Applications and Workflow
  • PCR Optimization: Reaction Conditions and Components (123.8KB)
  • Quantitative Analysis of the PCR Reaction (81.6 KB)
  
  
•  Thermal Cycler and Software Guides
  • GeneAmp® PCR System 2700 and 2720
  • GeneAmp® PCR System 9700
  • Veriti™ Thermal Cycler
  
  

 Protein Quantification

• Cleavable ICAT® Reagent Kit Troubleshooting Guide (408k)
  

 Protein Sequencing

• Procise® Cartridge Sensor Error Troubleshooting Tree (49k)
• Procise® PTH Chromatography Troubleshooting Tree (50k)
• Procise® PTH Computer Error Message Troubleshooting Tree (39k)
• Procise® Flask Sensor Errors Troubleshooting Tree (54k)
  

 Real-Time PCR

•  Getting Started with Real-Time PCR
  • New to Real-Time PCR? Start here!
  • Essentials of Real-Time PCR (153k)
  • Real-Time PCR: Understanding CT (2.03MB )
  • Applied Biosystems Real-Time PCR Rapid Assay Development Guidelines (329k)
  
  
•  Training
  • Real-Time PCR Training
  • Training Calendar
  • Self-Paced Training
  • Video Demonstrations and Pre-recorded Training
  • Product Webinars
  • Loading and Sealing Taqman Arrays
  • Register for a Taqman® OpenArray® Genotyping System Training CD
  
  
•  Applications and Workflow
  • Relative Gene Expression Workflow (2.5 MB PDF)
  • Chemistry Guide: Applied Biosystems Real-Time PCR Systems (1.74 MB)
  • Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative (1 MB)
  • Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR (287k)
  
  
•  Genomic Assays, Primer and Probe Design
  • Searching and Ordering Guide for TaqMan® Gene Expression Assays (2.24 MB)
  • Bioinformatic Evaluation of a Sequence for Custom TaqMan® Gene Expression Assays (1.5 MB)
  • Bioinformatic Evaluation of a Sequence for Custom TaqMan® SNP Genotyping Assays (4.31 MB)
  • TaqMan® Drug Metabolism Genotyping Assays: Reference Guide (2.3 MB)
  • Reconstituting and Diluting Primers and TaqMan® Probes (101k)
  • Load, Centrifuge and Seal TaqMan® Arrays (Flash Movie)
  • User Bulletin: Running TaqMan® Custom Arrays on 7900HT Real-Time PCR Systems: Applied Biosystems TaqMan® Custom Array: Rev A 873KB
  • Primer Express® Software
  
  
•  Instrument and Software Guides
  • StepOne™ and StepOnePlus™ Systems
  • 7300, 7500, and 7500 Fast Systems (SDS v1.4)
    
  •  7500 and 7500 Fast Systems (7500 Software v2.0, 2.1)
    • User Bulletin: Memory Upgrade Requirements for 7500 Software v2.0 and v2.0.1 (144 KB)
    • Standard Curve Experiments (91 MB)
    • Relative Standard Curve and Comparative CT Experiments (15.5 MB)
    • Genotyping Experiments (9.2 MB)
    • Presence/Absence Experiments (10.75 MB)
    • Computer Setup Guide (530 MB)
    
    
  •  7700 Sequence Detection System
    • Setting-up a Multiplex (two probe) Real Time 5' Nuclease Reaction on the ABI PRISM® 7700 Sequence Detection System (PDF, 410 KB)
    • Generating ABI PRISM® 7700 SDS Standard Curve Plots in a Spreadsheet Program (PDF, 317 KB)
    • Data Analysis on the ABI PRISM® 7700 SDS: Setting Baselines and Thresholds (PDF, 312 KB)
    • Generating ABI PRISM® 7700 SDS Amplification Plots in a Spreadsheet Program
    
    
  •  7900HT Fast System
    • Relative Quantitation Using Comparative CT (5.06 MB)
    • Allelic Discrimination (8.40 MB)
    • Absolute Quantitation Using Standard Curve (4.65 MB)
    • Plus/Minus Assay (7.89 MB)
    • Presence/Absence Experiments (10.75 MB)
  •  ViiA™ 7 PCR System
    • Quick Reference Card (2.02 MB)
    • Application Guides (31.87 MB)