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Applied Biosystems 3730xl DNA Analyzer
Specifications
Sequencing Production Capacity
| Run Module |
Capillary Length to Detector (cm) |
Runs/ Day |
LOR* |
Phred Q20 bases/read |
Phred Q20 bases/day |
| Rapid |
36 |
40 |
550 |
500 |
1,920,000 |
| Standard |
36 |
24 |
700 |
650 |
1,497,600 |
| Long-Read |
50 |
12 |
> 1,000 |
> 800 |
> 921,600 |
* 98.5% basecalling accuracy, less than 2% N's, using pGEM-3Zf(+) as template.
Capillary Arrays and Separation Matrix
| Capillary Separation Distance (cm) |
Dimensions |
Polymer Consumed/run |
| 36 |
150 μm o.d./50 μm i.d. |
Approx. 200 μL |
| 50 |
150 μm o.d./50 μm i.d. |
Approx. 250 μL |
Reagents
- BigDye® Terminator v 1.1
- BigDye® Terminator v 3.0
- BigDye® Terminator v 3.1
Instrument configuration
- CE Instrument
- Computer and Flat Panel Monitor
- Installation Chemistry and Accessories
- Collection and Analysis Software
Computer Specifications
- Base Unit: Pentium® IV Processor 2.00 GHz/400 MHz
- Memory: 1 GB, PC 800 @ 400 MHz
- Hard Drive: 72 GB
- Operating System: Windows® 2000 Professional Edition
- Monitor: 17" Flat Panel
- DVD-ROM Drive
Integrated Plate Stacker
- Houses 16 sample plates at any time
- Accommodates 96-well and 384-well plates
- Accessible any time except when autosampler is moving
Sample Volumes
- For 384-Well Sample Plates: 5-30 μL
- For 96-Well Sample Plates: 10-50 μL
Plate Seal
- Septa
- Polypropylene heat seal (automatic onboard piercing)
Laser
Argon-ion multi-line, single mode laser: primary excitation lines 488 and 514.5 nm.
Operating Environment
- Ambient temperature: 18�C to 30�C
- Humidity: 20 to 80% (non-condensing)
Oven Temperature
Active temperature control between 18˚C to 70˚C
Electrical
- Main Power: 200-220 V or 230-240 V+/-10%, 50/60 Hz
- Power Rating: Maximum input of 2500 VA
- Circuit Current: Maximum of 15 amps.
The instrument requires a 30 amp receptacle to match one of the two power
cord configurations that ship with the system. The electrical receptacle must
be located within 3 m (10 ft.) of the back of the instrument. Do not use extension
chords.
- Maximum Power Dissipation: 2,000 watts (approximation)
Dimensions (electrophoresis unit)
- Width: 100 cm
- Depth: 73 cm
- Height: 89 cm
- Weight: approximately 180 kg
Windows is a registered trademark of Microsoft
Corporation.
Pentium is a registered trademark of Intel Corporation
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