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Tutorials, Maintenance & Troubleshooting

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BioChromatography

BioCAD pumps and filters (219k PDF)

Busy Researchers Guide (9.1 MB PDF)

 

DNA Sequencing/Genotyping

ABI PRISM 310 Genetic Analyzer

310 Preferences for Macintosh (246k PDF)

310 Preferences for Windows (211k PDF)

Autosampler Calibration (635k PDF)

Clear Memory Reset (911k PDF)

Resolving Communication Problems (1 MB PDF)

Test CCD 4 Color and Seq Fill Modules (840k PDF)

310 Errors (780k PDF)

ABI PRISM® 3700 DNA Analyzer

ABI PRISM® 3700 Instrument -Hardware-Raw Data Troubleshooting Module

ABI PRISM® 3700 Instrument -Software-Extracting run data

ABI PRISM® 3700 Instrument -Troubleshooting Status Lights

Array View Colors Module (1.3 MB PDF)

Array View Module (372k PDF)

Checking CCD Window Module (310k PDF)

Checking for Bubbles Module (360k PDF)

Configuring the 3700Boot User Module (273k PDF)

Checking for Leaks Module (610k PDF)

Data Extraction Troubleshooting Module (511k PDF)

Electrophoresis Module (839k PDF)

Hardware Error Messages (743k PDF)

Instrument Maintenance Module (633k PDF)

Missing Lanes Module (246k PDF)

Raw Data Troubleshooting Module (1.63 MB PDF)

Running Service Modules (124k PDF)

Spatial Troubleshooting Module (1.71 MB PDF)

Status Lights Troubleshooting Module (708k PDF)

Verifying Syringe Pumps Module (622k PDF)

Verifying the Robot Module (555k PDF)

SeqScape® Software

Applied Biosystems SeqScape® Software v2.5: Quick Reference Guide: Rev A (333k PDF)

ABI PRISM® SeqScape® Software Version 2.x Tutorial: Rev B

SeqScape® Software Version 2.5: Frequently Asked Questions: Rev A (334k PDF)

 

PCR Applications

PCR Optimization: Reaction Conditions and Components (123.8 KB PDF)

Quantitative Analysis of the PCR Reaction (81.6 KB PDF)

 

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Protein Quantification

Cleavable ICAT® Reagent Kit Troubleshooting Guide (408k PDF)

 

Protein Sequencing

Procise® Cartridge Sensor Error Troubleshooting Tree (49k PDF)

Procise® PTH Chromatography Troubleshooting Tree (50k PDF)

Procise® PTH Computer Error Message Troubleshooting Tree (39k PDF)

Procise® Flask Sensor ErrorsTroubleshooting Tree (54k PDF)

 

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Real-Time PCR: Applications & Reagents

Getting Started with Real-Time PCR

Real-Time PCR Vs. Traditional PCR (969k PDF)

Essentials of Real Time PCR (153k PDF)

Real-Time PCR Rapid Assay Development Guidelines (1.06 MB)

Applications

Chemistry Guide: Applied Biosystems Real-Time PCR Systems (1.74 MB PDF)

Performing Relative Quantitation of Gene Expression Using Real-Time Quantitative PCR (20 MB PDF)

TaqMan® Genomic Assays

Searching and Ordering Guide for TaqMan® Gene Expression Assays (2.24 MB PDF)

Bioinformatic Evaluation of a Sequence for Custom TaqMan® Gene Expression Assays (1.5 MB PDF)

Bioinformatic Evaluation of a Sequence for Custom TaqMan® SNP Genotyping Assays (4.31 MB PDF)

TaqMan® Drug Metabolism Genotyping Assays: Reference Guide (2.3 MB PDF)

Primer and Probe Design

Primer Express® Tabs and Annotation Tools: An Overview of the features of Primer Express® v2.0

Primer Express® Software v2.0: Designing Primers and TaqMan® TAMRA™ Probes for 5' Nuclease Assays and Primers for Real-Time PCR Assays using SYBR® Green Dye

Designing TaqMan® MGB Probe and Primer Sets for Gene Expression Using Primer Express® Software Version 2.0 (440k PDF)

Designing TaqMan® MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express® Software Version 2.0 (436k PDF)

Primer Express® Software Version 3.0: Getting Started Guide (3.44 MB PDF)

Reagent Preparation

Reconstituting and Diluting Primers and TaqMan® Probes (101k PDF)

Creating Standard Curves with Genomic DNA or Plasmid DNA Templates for Use in Quantitative PCR (287k PDF)

 

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Real-Time PCR: Instrument Setup, Analysis & Maintenance

Applied Biosystems StepOne™ Real Time PCR System

Getting Started Guides

Relative Standard Curve and Comparative CT Experiments (11.51 MB PDF)

Genotyping Experiments (8.77 MB PDF)

Standard Curve Experiments (7.76 MB PDF)

Presence / Absence Experiments (9.7 MB PDF)

Reagent Guide (2.97 MB PDF)

Maintenance

Installation, Networking and Maintenance Guide (9.44 MB PDF)

Site Preparation Guide (1.24 MB PDF)

Applied Biosystems 7300/7500 Real Time PCR System

Getting Started Guides

Relative Quantification (3.85 MB PDF)

Allelic Discrimination (5.08 MB PDF)

Absolute Quantification (3.74 MB PDF)

Plus/Minus Assay (5.43 MB PDF)

Maintenance

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System: Installation and Maintenance Guide (15.3 MB PDF)

Site Preparation Guide (1.77 MB PDF)

Applied Biosystems 7900HT Real Time PCR System

Getting Started Guides

Relative Quantitation Using Comparative CT (5.06 MB PDF)

Allelic Discrimination (8.40 MB PDF)

Absolute Quantitation Using Standard Curve (4.65 MB PDF)

Plus/Minus Assay (7.89 MB PDF)

Maintenance

Maintenance and Troubleshooting Guide (14.70 MB PDF)

ABI PRISM® 7700 Sequence Detection System

Setup

Setting-up Singleplex (single probe) Real Time 5' Nuclease Reactions on the ABI PRISM® 7700 Sequence Detection System

Setting-up a Multiplex (two probe) Real Time 5' Nuclease Reaction on the ABI PRISM® 7700 Sequence Detection System (410k PDF)

Setting-up Real-Time PCR Assays using SYBR® Green Dye on the ABI PRISM® 7700 Sequence Detection System

ABI PRISM® 7700 SDS Dissociation Curve Set-Up Quick Guide (330k PDF)

Analysis

Generating ABI PRISM® 7700 SDS Standard Curve Plots in a Spreadsheet Program (317k PDF)

Data Analysis on the ABI PRISM® 7700 SDS: Setting Baselines and Thresholds (312k PDF)

Generating ABI PRISM® 7700 SDS Amplification Plots in a Spreadsheet Program

Maintenance

ABI PRISM® 7700 Sequence Detection System: Routine Maintenance

 

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Sample Preparation Systems

ABI PRISM™ 6100 Nucleic Acid PrepStation: Selecting Appropriate Protocols, Reagents and Consumables (203k PDF)

 

Thermal Cyclers

GeneAmp® PCR System 480:

GeneAmp® PCR System 480 Retirement Information (87k PDF)

GeneAmp® PCR System 2400:

Clearing User-Protected Stored Files from Applied Biosystems GeneAmp® PCR Thermal Cyclers (107k PDF)

GeneAmp® PCR System 2400 Coolant Replacement Procedure (537k PDF)

GeneAmp® PCR System 2400 Routine Maintenance (75k PDF)

GeneAmp® PCR System 2400 Retirement Information (90k PDF)

GeneAmp® PCR System 2700:

Clearing User-Protected Stored Files from Applied Biosystems GeneAmp® PCR Thermal Cyclers (107k PDF)

GeneAmp® PCR System 2700 Routine Maintenance (74k PDF)

Re-entering Lost Calibration Values on the GeneAmp® PCR System 2700 (390k PDF)

GeneAmp® PCR System 9600:

Clearing User-Protected Stored Files from Applied Biosystems GeneAmp® PCR Thermal Cyclers (107k PDF)

GeneAmp® PCR System 9600 Coolant Replacement Procedure (309k PDF)

GeneAmp® PCR System 9600 Routine Maintenance (70k PDF)

GeneAmp® PCR System 9600 Retirement Information (99k PDF)

GeneAmp® PCR System 9700:

Clearing User-Protected Stored Files from Applied Biosystems GeneAmp® PCR Thermal Cyclers (107k PDF)

GeneAmp® PCR System 9700 Routine Maintenance (12k PDF)

Re-entering Lost Calibration Values on the GeneAmp® PCR System 9700 (508k PDF)

 

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