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Absolute Quantitation

  1. Arnold, B.A., Hepler, R.W., and Keller, P.M. One-Step Fluorescent Probe Product-Enhanced Reverse Transcriptase Assay. BioTechniques 25(1):98-106, 1998.

  2. Ambs S., S. Dennis, J. Fairman, M. Wright and J. Papkoff. 1999. Inhibition of tumor growth correlates with the expression level of a human angiostatin transgene in transfected B16F10 melanoma cells [In Process Citation]. Cancer Res. 59:5773-5777, 1999.

  3. Becker K., D. Pan and C.B. Whitley. 1999. Real-time quantitative polymerase chain reaction to assess gene transfer. Hum. Gene Ther. 10:2559-2566, 1999.

  4. Berg, T., M ller, A.R., Platz, K.P., Hohne, M., Bechstein, W.O., Hopf, U., Wiedenmann, B., Neuhaus, P., and Schreier, E. Dynamics of GB virus C viremia early after orthotopic liver transplantation indicates extrahepatic tissues as the predominant site of GB virus C replication. Hepatology 29(1):245-249, 1999.

  5. Chiang, P.W., Wei, W.L., Gibson, K., Bodmer, R., and Kurnit, D.M. A fluorescent quantitative PCR approach to map gene deletions in the Drosophila genome [In Process Citation]. Genetics 153(3):1313-1316, 1999.

  6. de Kok, J.B., Hendriks, J.C., van Solinge, W.W., Willems, H.L., Mensink, E.J., and Swinkels, D.W. Use of real-time quantitative PCR to compare DNA isolation methods. Clin.Chem. 44(10):2201-2204, 1998.

  7. Fairman, J., Roche, L., Pieslak, I., Lay, M., Corson, S., Fox, E., Luong, C., Koe, G., Lemos, B., Grove, R., Fradkin, L., and Vernachio, J. Quantitative RT-PCR to evaluate in vivo expression of multiple transgenes using a common intron [In Process Citation]. BioTechniques 27(3):566-70, 572-4, 1999.

  8. Gerard, C. J., et al. "Improved quantitation of minimal residual disease in multiple myeloma using real-time polymerase chain reaction and plasmid-DNA complementarity determining region III standards." Cancer Res 58 (1998): 3957-3964.

  9. Hartel, C., Bein, G., Kirchner, H., and Kluter, H. A Human Whole-Blood Assay for Analysis of T-Cell Function by Quantification of Cytokine mRNA. Scand.J.Immunol. 49(6):649-654, 1999.

  10. Higgins, J. A., et al. "5' nuclease PCR assay to detect Yersinia pestis [In Process Citation]." J Clin Microbiol 36 (1998): 2284-2288.

  11. Hennig, H., et al. "Lack of evidence for Marek's disease virus genomic sequences in leukocyte DNA from multiple sclerosis patients in Germany [In Process Citation]." Neurosci Lett 250 (1998): 138-140.

  12. Houng, H.H., Hritz, D., and Kanesa-thasan, N. Quantitative detection of dengue 2 virus using fluorogenic RT-PCR based on 3'-noncoding sequence. J.Virol.Methods 2000.Apr;86(1):1-11.

  13. Kafert S., J. Krauter, A. Ganser and M. Eder. Differential quantitation of alternatively spliced messenger RNAs using isoform-specific real-time RT-PCR. Anal. Biochem. 269:210-213, 1999.

  14. Kimura, H., Morita, M., Yabuta, Y., Kuzushima, K., Kato, K., Kojima, S., Matsuyama, T., and Morishima, T. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J.Clin.Microbiol. 37(1):132-136, 1999.

  15. Klein, D., Janda, P., Steinborn, R., Muller, M., Salmons, B., and Gunzburg, W.H. Proviral load determination of different feline immunodeficiency virus isolates using real-time polymerase chain reaction: influence of mismatches on quantification [In Process Citation]. Electrophoresis 20(2):291-299, 1999.

  16. Lo, Y. M., et al. "Quantitative analysis of fetal DNA in maternal plasma and serum: implications for noninvasive prenatal diagnosis." Am J Hum Genet 62 (1998): 768-775.

  17. Lo, Y.M., Chan, L.Y., Lo, K.W., Leung, S.F., Zhang, J., Chan, A.T., Lee, J.C., Hjelm, N.M., Johnson, P.J., and Huang, D.P. Quantitative analysis of cell-free Epstein-Barr virus DNA in plasma of patients with nasopharyngeal carcinoma. Cancer Res. 59(6):1188-1191, 1999.

  18. Lo, Y.M., Leung, T.N., Tein, M.S., Sargent, I.L., Zhang, J., Lau, T.K., Haines, C.J., and Redman, C.W. Quantitative abnormalities of fetal DNA in maternal serum in preeclampsia [see comments]. Clin.Chem. 45(2):184-188, 1999.

  19. Lo Y.M., L.Y. Chan, A.T. Chan, S.F. Leung, K.W. Lo, J. Zhang, J.C. Lee, N.M. Hjelm, P.J. Johnson and D.P. Huang. 1999. Quantitative and temporal correlation between circulating cell-free Epstein-Barr virus DNA and tumor recurrence in nasopharyngeal carcinoma. Cancer Res. 59:5452-5455.

  20. Leutenegger, C.M., Klein, D., Hofmann-Lehmann, R., Mislin, C., Hummel, U., Boni, J., Boretti, F., Guenzburg, W.H., and Lutz, H. Rapid feline immunodeficiency virus provirus quantitation by polymerase chain reaction using the TaqMan fluorogenic real-time detection system [In Process Citation]. J.Virol.Methods 78(1-2):105-116, 1999

  21. Lockey, C., E. , Otto, and Z. , Long. "Real-time fluorescence detection of a single DNA molecule." BioTechniques 24 (1998): 744-746.

  22. Luthra, R., et al. "Novel 5' exonuclease-based real-time PCR assay for the detection of t(14;18)(q32;q21) in patients with follicular lymphoma." Am J Pathol 153 (1998): 63-68.

  23. Maudru, T. and K. W., Peden. "Adaptation of the fluorogenic 5'-nuclease chemistry to a PCR-based reverse transcriptase assay." BioTechniques 25 (1998): 972-975.

  24. Nagai, M., Usuku, K., Matsumoto, W., Kodama, D., Takenouchi, N., Moritoyo, T., Hashiguchi, S., Ichinose, M., Bangham, C.R., Izumo, S., and Osame, M. Analysis of HTLV-I proviral load in 202 HAM/TSP patients and 243 asymptomatic HTLV-I carriers: high proviral load strongly predisposes to HAM/TSP. J.Neurovirol. 4(6):586-593, 1998.

  25. Nogva, H.K. and Lillehaug, D. Detection and quantification of Salmonella in pure cultures using 5'-nuclease polymerase chain reaction [In Process Citation]. Int.J.Food Microbiol. 51(2-3):191-196, 1999.

  26. Pusterla, N., Huder, J.B., Leutenegger, C.M., Braun, U., Madigan, J.E., and Lutz, H. Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks. J.Clin.Microbiol. 37(5):1329-1331, 1999.

  27. Pusterla, N., Leutenegger, C.M., Chae, J.S., Lutz, H., Kimsey, R.B., Dumler, J.S., and Madigan, J.E. Quantitative Evaluation of Ehrlichial Burden in Horses after Experimental Transmission of Human Granulocytic Ehrlichia Agent by Intravenous Inoculation with Infected Leukocytes and by Infected Ticks. J.Clin.Microbiol. 37(12):4042-4044, 1999.

  28. Smith, G.J., et al. "Fast and accurate method for quantitating E. coli host-cell DNA contamination in plasmid DNA preparations." BioTechniques 26 (1999): 518-526.

  29. Wang, S.C., Klein, R.D., Wahl, W.L., Alarcon, W.H., Garg, R.J., Remick, D.G., and Su, G.L. Tissue coexpression of LBP and CD14 mRNA in a mouse model of sepsis. J.Surg.Res. 76(1):67-73, 1998.

  30. Wang, T. and Brown, M.J. mRNA Quantification by Real Time TaqMan Polymerase Chain Reaction: Validation and Comparison with RNase Protection [In Process Citation]. Anal.Biochem. 269(1):198-201, 1999.


  32. Wang X., X. Li, J.A. Erhardt, F.C. Barone and G.Z. Feuerstein. 2000. Detection of tumor necrosis factor-alpha mRNA induction in ischemic brain tolerance by means of real-time polymerase chain. J. Cereb. Blood Flow Metab. 2000. Jan;20(1):15-20. 20:15-20.

  33. Wang X., X. Li, R.W. Currie, R.N. Willette, F.C. Barone and G.Z. Feuerstein. 2000. Application of real-time polymerase chain reaction to quantitate induced expression of interleukin-1beta mRNA in ischemic brain tolerance. J. Neurosci. Res. 2000. Jan. 15;59(2):238-46.

  34. Ying H., T.Z. Zaks, R.F. Wang, K.R. Irvine, U.S. Kammula, F.M. Marincola, W.W. Leitner and N.P. Restifo. Cancer therapy using a self-replicating RNA vaccine. Nat. Med. 5:823-827, 1999.